APPROACHES FOR BIOLOGICAL IMPROVEMENT OF ECO-FRIENDLY CROPS
Journal Title: Journal of Biological and Scientific Opinion - Year 2013, Vol 1, Issue 3
Abstract
Marker gene is a DNA sequence whose phenotype can be easily recognized and identified. It can be used to flag another gene. A selective marker encourages the growth in the presence of a selective agent, which kills or prevents the growth of a cell. Antibiotics resistance gene is used to tag target gene or gene carrying a trait of interest. Antibiotics are used in crops during transfer of gene of interest in crops improvement programmes. Antibiotics and antibiotics resistance gene has been used for a long time in plant biotechnology. Not even one Gene transfer method has so much efficiency so that screening allows the selection of transformed cells having marker gene together with target gene which inactivates the antibiotic and protect the cells from antibiotic while non transformed cells do not have marker gene so they are killed in presence of antibiotics during screening.
Authors and Affiliations
Siddharth Priyadarshi , Anubha Shukla , Babasaheb Borse
Keywords
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- EP ID EP120629
- DOI 10.7897/2321–6328.01325
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