Artifactual Changes in Whole Blood and Plasma Glucose Levels of Diabetic and Non Diabetic Blood Samples Twenty Four Hours (24 h) Post Collection
Journal Title: International Blood Research & Reviews - Year 2016, Vol 5, Issue 3
Abstract
Analysis of diabetic blood may be delayed for hours post collection owing to several factors. Artifactual changes may occur thereby confounding the results. This study investigated some artifactual changes in glucose levels that may occur in diabetic blood stored at room temperature for 24 h. Ten (10) male Wistar rats were assigned to two (2) groups of five (5) rats per group. Group 1 rats were made diabetic by single intraperitoneal injection of 160 mg/kg of alloxan monohydrate while group 2 rats served as normal control. Rats with blood glucose values ≥ 126 mg/dl were considered diabetic. One week (7 days), following establishment of diabetes, blood samples were collected after overnight fasting from both diabetic and non diabetic rats using heparinized capillary tubes into sample bottles. Determination of the blood glucose values were done 1 h post collection and subsequently after every 2 h for 24 h on both whole blood and plasma. Results indicated earlier significant (p<0.05) decreases in the glucose values of diabetic whole blood samples compared to the non-diabetic counterpart. Decreases in the glucose levels of whole blood sample were significantly (p<0.05) higher compared to that of the plasma. It was concluded that the blood and plasma sugar levels of diabetic rats deteriorated faster compared to the non diabetic counterpart and that significant changes in the glucose levels of both blood and plasma occurred within 2 h post collection. The plasma sugar levels of non diabetic rats were unreliable (increased rather than decrease).
Authors and Affiliations
Patrick Emeka Aba, Miracle Nnaemeka Edeh, Ernest Chukwuamachago Nweze
Keywords
Related Articles
- EP ID EP242618
- DOI 10.9734/IBRR/2016/26044
- Views 155
- Downloads 0
Stem Cell Diversity and Applications in Therapy
Aim: In this review, we will discuss the different types of stem cells and their uses in therapies. Introduction: Stem cells are cells produced during pregnancy, and have the ability to differentiate and self renew via s...
Complications of Allogeneic Blood Transfusion: Current Approach to Diagnosis and Management
Introduction: Blood transfusion remains a vital component of modern medicine, as yet artificial blood or blood substitute is still widely promising. In well-organized health care systems, with standard transfusion servic...
Cytomegalovirus-induced Pure Red Cell Aplasia Successfully Treated with Ganciclovir: A Case Report
Background: Acquired pure red cell aplasia (PRCA) is a rare disease characterized by anemia, severe reticulocytopenia and absent to low bone marrow erythroid precursor cells. There are well-described associations of this...
Palliative Radiotherapy for Spinal Extramedullary Hematopoiesis in Thalassemia Major
Introduction: Non-hepatosplenic Extramedullary Hematopoiesis (NHEMH) is seen as a compensation mechanism in the patients with hematologic dysfunction. Thalassemia is an autosomal recessive hematologic disorder. The tissu...
Storage of Blood with or without Irradiation: Effect on Lipid Profile
Aims: To observe the effect of storage on lipid profile in pre-irradiated blood and non-irradiated blood before transfusion. Study Design: Prospective study. Place and Duration of Study: Department of Biochemistry and D...