Assesment of different DNA extraction methods in medicinal plants

Journal Title: Journal of Herbal Drugs - Year 2011, Vol 2, Issue 1

Abstract

Medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. Essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. These compounds especially antioxidants can increase oxidation of DNA in response to wounds. Nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationships, construction of linkage maps and decreasing the repeated plants in gene bank. Most of these methods need high DNA quality and purity. Different methods for DNA extraction have been improved. In most cases three hints are considered including quality, quickness of extraction and its quantity. Medicinal plants need to improve methods to prevent DNA oxidation because of their phenolic compounds. In this study, four DNA extraction methods were compared in thyme, spearmint, peppermint, horsemint and some yarrow species. These methods include Murry and Thompson, Prittila et al, Dellaporta et al and Komatsuda et al method using blender. The results showed that the combination of Prittila et al and Murry and Thompson methods using two steps buffer and using mixed of 2- Mercaptoethanol and Polyvinylpyrrolidone (PVP) can decrease DNA oxidation and improve DNA amount. The lowest amount of DNA and the quickest method was Komatsuda et al procedure. In the studied plants, the highest and the lowest oxidation were observed in yarrow and spearmint, respectively.

Authors and Affiliations

Mehdi Rahimmalek

Keywords

Related Articles

Investigation of input energy efficiency in dill (Anethum graveolens L.) farming in Rodehen region

Background & Aim: Energy is one of important debates in agriculture ecology and for this reason; the ratio of output and input energy in different agriculture ecosystems has been calculated. If one can reduce, input ener...

Performance development of broiler chickens fed diets containing the seeds of Silybum marianum L. and Thymus vulgaris L.

Background & Aim: Medicinal plants is very potential to be used as a supplementary feed as well as feed additive in broilers. This experiment was conducted to investigate the effects of milk thistle (MT) and thyme see...

Effect of Cichorium intybus L. extracts and Kelussia oderatassima Mozaff. essential oil on toxic of organophosphouros insecticides in RAT

Background & Aim: Organophosphates are frequently used as insecticides in households, in agricultural areas and veterinary, thus posing a risk for accidental dermal exposure or through inhalation. Acute organophosphate p...

The effect of climatic conditions on the concentration of stevioside in Stevia production in different regions of Iran and optimization of stevioside extraction

Background & Aim: According to the increase in diseases such as diabetes and obesity, using of saccharose alternatives for producing of low-calorie and sugar-free alternatives, are taken into consideration of researchers...

Chemical components of essential oils of four Eucalyptus L'Her. species cultivated in Kashan’s botanical garden, Iran

Background & Aim: Eucalyptus L'Her. [an aromatic genera of the family (Myrtaceae)] is native to Australia and other tropical species is planted worldwide. The purpose of this study is to extract and identify the essentia...

Download PDF file
  • EP ID EP23336
  • DOI -
  • Views 310
  • Downloads 22

How To Cite

Mehdi Rahimmalek (2011). Assesment of different DNA extraction methods in medicinal plants. Journal of Herbal Drugs, 2(1), -. https://europub.co.uk/articles/-A-23336