Assessment of biodegradability and cytotoxicity of mPEG-PCL diblock copolymers and PCL-PEG-PCL tri block copolymers on HEK293 cells
Journal Title: مجله علمي دانشگاه علوم پزشكي كردستان - Year 2017, Vol 22, Issue 89
Abstract
Background and Aim: Knowledge of biodegradability and biocompatibility rate in drug delivery systems is very important. Material and Method: This study PCL–PEG–PCL and MPEG-PCL copolymers were synthesized by ring-opening copolymerization. HNMR, FTIR, DSC and GPC techniques were used to confirm successful synthesis and determination of the characteristics of the copolymers. We evaluated biocompatibility by MTT assay on the basis of cytotoxicity effect of these copolymers on HEK293 cells. Biodegradation was evaluated by GPC technique. Results: The results of HNMR and FTIR confirmed synthesis of diblock and tri block copolymers. Using DSC technique, melting point of tri block and diblock copolymers were 55 °C and 58.68°C and also the average molecular weights of these copolymers were 16,000 and 12,223 Dalton, respectively. The degradation of these copolymers were performed in buffer solution and at the end of the 31st day, molecular weights of diblock and tri block Copolymers were 7852 and 9586 Dalton respectively. Based on IC50 , diblock and tri block copolymers had no toxic effects on HEK293 cells and are biocompatibile . Conclusion: diblock and tri block copolymers had high rates of biodegradability and because of not having toxic effects on normal renal cells, they were biocompatible.
Authors and Affiliations
PhD H Kheiri Manjilli, PhD A Sharafi
Keywords
Related Articles
- EP ID EP282916
- DOI -
- Views 101
- Downloads 0
The effect of proprioceptive neuromuscular facilities (PNF) stretching exercise on patellofemoral pain syndrome (PFPS)
Background and Aim: Patellofemoral pain syndrome (PFPS) is a common disorder of the knee. the conservative treatment of patellofemoral pain syndrome is to correct shortness of soft tissue. The aim of this study was to d...
Serum procalcitonin level as a biomarker for the differential diagnosis between infectious and non-infectious systemic inflammatory response syndrome: a prospective case–control study
Background and Aim: Considering the importance of sepsis and its complications in the mortality of the patients admitted to different parts of hospital and the importance of early diagnosis and timely and appropriate tre...
The effect of endurance activity on miR-499 and sox6 genes expression in fast and slow twitch skeletal muscles
Background and Aim: Endurance activity can affect function and also factors involved in skeletal muscle transcription. The aim of this study was to investigate the effects of an endurance activity program on the Sox6 gen...
Epidemiological study of irritable bowel syndrome and its related factors in Sanandaj from 2013 to 2014: a population-based study
Background and Aim: Irritable bowel syndrome is a common gastrointestinal disease which is characterized by abdominal pain and altered bowel habits. This study aimed to investigate the prevalence of irritable bowel syndr...
The relationship between hemoglobin level in the first trimester of pregnancy and preterm delivery
Background and Aim: Anemia is the most prevalent nutritional deficiency in the world, affecting almost 30% of people worldwide, especially the pregnant women. On the other hand preterm birth is responsible for 2/3 of th...