Assessment of biodegradability and cytotoxicity of mPEG-PCL diblock copolymers and PCL-PEG-PCL tri block copolymers on HEK293 cells
Journal Title: مجله علمي دانشگاه علوم پزشكي كردستان - Year 2017, Vol 22, Issue 89
Abstract
Background and Aim: Knowledge of biodegradability and biocompatibility rate in drug delivery systems is very important. Material and Method: This study PCL–PEG–PCL and MPEG-PCL copolymers were synthesized by ring-opening copolymerization. HNMR, FTIR, DSC and GPC techniques were used to confirm successful synthesis and determination of the characteristics of the copolymers. We evaluated biocompatibility by MTT assay on the basis of cytotoxicity effect of these copolymers on HEK293 cells. Biodegradation was evaluated by GPC technique. Results: The results of HNMR and FTIR confirmed synthesis of diblock and tri block copolymers. Using DSC technique, melting point of tri block and diblock copolymers were 55 °C and 58.68°C and also the average molecular weights of these copolymers were 16,000 and 12,223 Dalton, respectively. The degradation of these copolymers were performed in buffer solution and at the end of the 31st day, molecular weights of diblock and tri block Copolymers were 7852 and 9586 Dalton respectively. Based on IC50 , diblock and tri block copolymers had no toxic effects on HEK293 cells and are biocompatibile . Conclusion: diblock and tri block copolymers had high rates of biodegradability and because of not having toxic effects on normal renal cells, they were biocompatible.
Authors and Affiliations
PhD H Kheiri Manjilli, PhD A Sharafi
Keywords
Related Articles
- EP ID EP282916
- DOI -
- Views 111
- Downloads 0
Evaluation of oxidant effect of aqueous extract of Ruta graveolens on mice ovary
Background and Aim:Ruta Graveolens (RG) commonly called Sedab, has been known as a medical plant since ancient times. Flavonoids, rutin (quercetin-rutinoside), quercetin, and furanocoumarins are the most common chemic...
Nurses’ mental health in Iran: A national survey in teaching hospitals
Background and Aim: Mental health disorders are common problems among health care professionals such as nurses, which can create great problems .The aim of this study was to evaluate mental health status in nursing perso...
XML Expression of aap and icaR genes involved in biofilm production in clinical strains of Staphylococcus aureus resistant to methicillin and gentamicin
Backgrounds and Aim: Staphylococcus aureus biofilms are involved in a multitude of serious chronic infections. Production of biofilms is a defensive-invasive process controlled and regulated by the aap and icaR genes. Th...
Effectiveness and complications of ESWL in the treatment of renal pelvis stones in the children less than 8 years of age in Sanandaj, Iran 2007-2015
Background and Aim: Extracorporeal shock wave lithotripsy (ESWL) has revolutionized the treatment of kidney stones in children. However, use of this technology has several complications which cast doubt on its safety and...
Anxiolytic and antidepressant effects of cinnamon (Cinnamomum verum) extract in rats receiving lead acetate
Background and Aim: Lead is one of the environmental pollutants that enters the nervous system and leads to neurological disorders such as depression and anxiety. Cinnamon (Cinnamomum verum) has antioxidant, anti-inflamm...