Assessment of contamination levels and characterization of Aflatoxin B1 producing Aspergillus flavus strain from food and feed samples from local markets of south India
Journal Title: International Journal of Life Science - Year 2018, Vol 6, Issue 2
Abstract
Aflatoxins are the toxic secondary metabolites produced by fungi Aspergillus flavus and A. parasiticus in many food and feed products including oil seeds when stored under ambient conditions. Aflatoxin B1 a prototype of the aflatoxin is widely recognized as group I human hepato-carcinogenic compound by IARC. Contamination of oil seeds with the Aspergillus Sps and aflatoxin possess serious threat to the food and feed industry. The present study on the incidence of seed borne infection and evaluation of aflatoxin B1 contamination levels in oil seeds by applying the principles of standard blotter method and chromatography methods respectively. The molecular characterization of toxigenic isolate No.39 by PCR method. The percentage incidence of seed borne infection was observed to be higher for the groundnut sample showing the mean percentage of infection to be about 48.57% than with all the seed samples. Among 55 isolates of Aspergillus flavus, 42 were found to be aflatoxigenic strains by TLC and amount of aflatoxin B1 in oil seeds was found to be in the range of 29-35µg/ml by HPLC. The molecular characterization of toxigenic isolate was found to be closely related to Aspergillus flavus strain DAOM 225949 and the nucleotide sequence was provided with a GenBank accession number JN938987. This study concluding that the oil seeds were prone to fungal infection and contamination with aflatoxin B1 when stored under ambient conditions. So, proper storage, improvement of Good Agricultural Practices and regular inspection should be practiced ensuring that the safety of animals including human beings.
Authors and Affiliations
AS Manjunatha, K Manjunath, DC Mohana
Keywords
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