Assessment of disk diffusion and E-test methods to determine antimicrobial activity of cefalotin and vancomycin on clinical isolates of Staphylococcus aureus

Abstract

The present investigation aims to find a specific method to test the clinical isolates of Staphylococcus aureus in terms of antimicrobial susceptibility and resistance to two drugs of cefalotin and vancomycin using E-test and Disk Diffusion methods. Totally 80 samples were collected from hospitalized patients at the general hospitals of Kerman Province, Southeastern, Iran between November 2013 to April 2014. They were identified by standard microbiological methods. Susceptibility by Disk diffusion and MIC by E-test were performed according to the Clinical and Laboratory Standards Institute breakpoints. In the disk diffusion method for vancomycin antibiotic, the S. aureus specimens were reported to be 15% resistant, 27% intermediate, and only 38% susceptible. Also, for cefalotin antibiotic, the specimens were reported as 48% resistant, 19% intermediate, and only 13% susceptible. In the E-test method for vancomycin antibiotic, the S. aureus specimens were reported to be 29% resistant, 18% intermediate, and 33% susceptible. Also, for cefalotin, the specimens were reported to be 59% resistant, 11% intermediate, and 10% susceptible. By statistical comparison using SPSS software and Chi Square test, the P-value of 0.001 was obtained; therefore, regarding the zero hypothesis, there is a significant relationship between these two methods of microbial susceptibility determination at (p <0.05), and accept that the E-test method is still a suitable test for susceptibility determination for this bacterium. The findings of present study revealed the high resistance of this bacterium in Kerman Province and feeling the necessity of thinking of some strategies and solutions for reducing that microbial resistance as well as paying more attention to the selective treatments, antibiotic treatment course duration, and other instances that should be taken into account in any antibiotic diet in order to prevent and avoid such high levels of microbial resistance in our country.

Authors and Affiliations

Mehdi Rezaeifar| Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran, Mohammad Bagher Bagheri| Department of Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran, Mohammad Moradi| Department of Medical Microbiology, Kerman University of Medical Sciences, Kerman, Iran, Mostafa Rezaeifar| Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran, Corresponding Email: mrezaeifar@gmail.com

Keywords

Related Articles

A Comparison between Quality of Life of the Patients under Methadone Maintenance Treatment with and without Alcohol abuse

Alcohol consumption during methadone maintenance treatment (MMT) is among the common challenges in the addiction field. It is reported that alcohol overuse is closely related to the peripheral consequences of social hea...

AN UNUSUAL FINDING OF EPIDIDYMAL SPERM GRANULOMA IN AN ORCHIDECTOMY SPECIMEN: A CASE REPORT

Epididymal sperm granuloma as a cause of granulomatous epididymitis is rare. Most of the cases are found post vasectomy as part of the late vasectomy syndrome. These lesions are known to mimic other conditions includin...

Acute kidney injury after open heart surgery in seyyed-al-Shohad heart center urmia(Iran)

Cardiac surgery-associated acute kidney injury (CSA-AKI)is a serious, well known medical problem with significant morbidity and marked predisposition to mortality in 9-45% of patients undergoing cardiac surgeries. Clini...

Comparison of Heavy- and Light-Weight Composite Mesh in Pain Reduction Among Patients with Inguinal Hernia: A 1-Year Randomized Control Trial

Background: Inguinal hernia is commonly addressed complication among men and women. Surgical mesh repair is widely used as a treatment. Postoperative chronic pain is a major complication and concern, as it restricts the...

ISOLATED GASTRIC TUBERCULOSIS MASQUERADING AS CHRONIC PEPTIC ULCER: A CASE REPORT

Abdominal Tuberculosis (TB) most commonly affects ileo-caecal region. Isolated stomach involvement by TB, without pulmonary infection is rare. Clinical presentation of Stomach TB may be non-specific, radiological find...

Download PDF file
  • EP ID EP12135
  • DOI -
  • Views 272
  • Downloads 12

How To Cite

Mehdi Rezaeifar, Mohammad Bagher Bagheri, Mohammad Moradi, Mostafa Rezaeifar (2016). Assessment of disk diffusion and E-test methods to determine antimicrobial activity of cefalotin and vancomycin on clinical isolates of Staphylococcus aureus. International Journal of Medical Research & Health Sciences (IJMRHS), 5(11), 122-126. https://europub.co.uk/articles/-A-12135