Assessment of genetic diversity in Colletotrichum capsici using Randomly Amplified Polymorphic DNA
Journal Title: IJAR-Indian Journal of Applied Research - Year 2015, Vol 5, Issue 1
Abstract
Present study was undertaken to estimate genetic diversity in Colletotrichum capsici causing anthracnose disease on chilli fruits, using Randomly Amplified Polymorphic DNA (RAPD). In this study collect 12 isolates of Colletotrichum capsici from different sites and grow in PDA media. After pathogenicity test fungal pathogens were inoculate in PD broth. Mycelium mat form that harvest after 14-21 days and DNA was isolate. RAPD analysis after PCR amplification. Six RAPD primers were use to amplify genomic DNA of 12 Colletotrichum capsici isolates. DNA fragments of various sizes ranging from 250 to 4000 bp were amplified. The isolates were divided into two major clusters based on dendrogram construction from UPGMA clustering. Some pathogen cluster were to be pathogenically diverse and the diversity seems to be due to continuous generation of molecular characterization. The analysis of pathogenic cluster according to geographic distribution of the pathogenic population.
Authors and Affiliations
Saket Kumar, Vineeta Singh, Major Singh
Keywords
Related Articles
- EP ID EP529212
- DOI -
- Views 97
- Downloads 0
Adjuvant Non Phamacotherapy With Tepid Sponging With Bath Warm Water To Reduce Duration & Severity of Viral Fevers
Tepid sponging therapy is practicing since olden days can be useful to reducing temperature . Fomentation means a warm application of water ,the therapeutic application of warmth and moisture as to relieve pain . 50 pati...
Some Results on Acyclic Chromatic Number of Mth Power of Paths
In this paper we discuss the graph power of paths, and some of their structural properties are derived. The acyclic chromatic number of Pn m and is derived both analytically and using their adjacency matrices. Also a rel...
British Literature: A Study of Traditional and Cultural Founding in The Waste Land By T.S.Eliot
T.S.Eliot had been very influential in shaping Modernist Poetry. He was instrumental in bringing about a innovative change in the language and images of the 20th century poetry. His poetic shorthand became inspirational...
Comparative Study Between Quincke’s (23G, 25G) and Whitacre (25G) Induced Post Dural Puncture Headache After Neuraxial Blockade in Lower Abdominal Surgeries
A Prospective, Randomized, Comparative Study was designed to evaluate the occurance of post dural puncture headache following the use of two different needles of same Guage (25G)with one pencil point needle(Whitacre) and...
EXTENSIVE PHYSIOLOGIC PIGMENTATION OF TONGUE
Pigmented lesions are commonly found in the oral cavity. Such lesions may present as physiologic discoloration, manifestations of systemic diseases or malignant neoplasms, therefore an understanding of mucosal pigmentati...