Assessment of genetic purity of inter-specific f1 hybrids involving vigna radiata and vigna umbellata
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Genetic purity test of true hybrids from controlled crosses before further generations of selfing or crossing and selection is essential for Mungbean improvement. The present study was conducted to transfer mungbean yellow mosaic (MYM) disease resistance in mungbean from ricebean and to assess the genetic purity of developed inter-specific F1 hybrids using morphological features and microsatellite markers. Inter-specific crosses were made involving two genotypes of mungbean viz., K 851 and TM 96-2 and one genotype of ricebean (RBL 1) where the crossability results revealed significant differences. Crossability was recorded 8.2% (TM 96-2 × RBL 1) and 4.6% (K 851 × RBL 1). Pollen fertility was recorded 1.6% and 3.4% in TM 96-2 × RBL 1 and K 851 × RBL 1, respectively. Morphological features such as epicotyl colour, hypocotyl length, petiole length, germination habit, etc., were used as indicators of true hybridity. Further, the microsatellite markers were used to confirm the genetic purity of the developed inter-specific hybrid. These hybrids exhibited resistance against mungbean yellow mosaic disease under natural epiphytotic field conditions. The present study will be useful in developing high yielding varieties or lines of mungbean coupled with stable MYMV resistance.
Authors and Affiliations
Akhouri Nishant Bhanu, Pankaj Kumar, Mahendra Narain Singh, Kartikeya Srivastava , Akhouri Hemantaranjan
Keywords
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- EP ID EP428133
- DOI 10.18006/2017.5(5).636.643
- Views 59
- Downloads 0
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