ASSESSMENT OF PATHOPHYSIOLOGY OF VITAMIN D DEFICIENT PATIENTS: A TERTIARY CARE CENTRE STUDY
Journal Title: Journal of Advanced Medical and Dental Sciences Research - Year 2017, Vol 5, Issue 6
Abstract
Background: There are two forms of vitamin D i.e. vitamin D1 and Vitamin D2. Vitamin D1 is obtained from dietary sources and vitamin D2 is synthesized on the exposure to UV light. Serum hydroxylated forms of vitamin D i.e. 25 OHD are the most reliable indicator of vitamin D status of a person. Even though India is a tropical country, receiving ample amount of sunlight, still deficiency has been reported by international osteoporosis foundation amongst all age groups. The aim of present study is to evaluate the levels of Vitamin D amongst patients of a tertiary care hospital and to estimate the impact of age and gender. Material And Methods: The present study was conducted among 1004 subjects over a period of 15 months. The test for estimation of vitamin D was a competitive test, where vitamin D of patient’s serum competes with biotin Vitamin D conjugate ( that was added simultaneously) for antivitamin D antibodies binding sites. In the end streptavidin HRP conjugate was added and then addition of TMB reagent detected the reaction. A duplicate test was run alongside with the controls provided by the kit, which acted as a quality control measure. The data collected was arranged in tabulated and graphical form. The results are expressed as mean+/- standard deviation. Results: Out of the total of 1004 subjects, females were in majority. The number of males was 455 and number of females was 549. Maximum levels of vitamin D were seen amongst 11-20 years of age i.e. 39.11 which were followed by 41-50 years of age. Conclusion: Majority of the subjects were deficient of Vitamin D. Only 26% subjects had sufficient levels of vitamin D. The mean levels of vitamin D were highest between 11-20 years of age.
Authors and Affiliations
Udita Singhal, Manoj Sareen, Pearly Sen
Keywords
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