Assessment of Tetracyclines Residues Contamination in Chicken Meat Samples of Algiers Slaughterhouses Level
Journal Title: Advances in Clinical Toxicology - Year 2023, Vol 8, Issue 4
Abstract
Food contamination with antimicrobial residues may occur, entailing assessment and monitoring of the contamination level in edible tissue. In that way, 151 chicken meat samples were randomly collected from Algiers slaughterhouses. Otherwise, two analytical methods are validated for the simultaneous determination of oxytetracycline, tetracycline, doxycycline and chlortetracycline residues in edible chicken tissues, by liquid chromatography combined with mass spectrometry detection. Beforehand, an extraction step is performed. Indeed, acetonitrile is used as an extraction solvent for the qualitative method, and then the quantitative method is carried out after a preliminary extraction with an acidified EDTA-McIlvaine buffer, followed by a solid-phase extraction cleanup. Validation qualitative method results were satisfactory in terms of specificity (100%), sensivity, detection capability (CCß) and detection limit (LOD). Furthermore, validation results of the quantitative method was pertinent to the EU commission criteria. Thus, determination coefficient (r2) values were between de 0.91 to 0.98%, where the lowest values were observed for doxycycline. Trueness was between 85.5% and 104.8, expect for tetracycline (76.6% at 150μg/kg) and doxycycline (134.7% at 100μg/kg). As for intraday and interday precision, values respectively ranged between 12.5 and 26% and between 14 and 35%, except doxycycline for which precision values were higher. The methods have been successfully used for the identification, confirmation and quantification of tetracyclines in chicken muscle samples. Consequently, 25 samples were suspects in regard tetracyclines residues. The comparison of these samples with the QC at MRL (100μg/kg) revealed five samples needed a quantification. Thus, oxytetracycline, its epimer and doxycycline identified in samples were quantified. Indeed, all samples contained oxytetracycline and its epimer, doxycycline or both showed levels less than the decision limit (CCα).
Authors and Affiliations
Zamoum R*, Baroudi F, Djerroud D, Khouni B, Alamir B
Keywords
Related Articles
- EP ID EP752294
- DOI 10.23880/act-16000280
- Views 9
- Downloads 0
Potential of the Combination of a Systemic Enzyme Complex and Probiotics administration to Combat COVID-19: A Randomized Open Label Prospective Analysis
Background: Enzymes have been used for therapeutic applications for decades owing to their anti-inflammatory and immunomodulatory effects. Probiotics are well known to reduce the incidence and severity of several health-...
Relationship between Working Clothes and Oxidative Stress in Agricultural Workers
Agricultural workers are frequently exposed to ultraviolet (UV) radiation, which causes various diseases by inducing oxidative stress. This study aimed to assess the effects of working clothes on oxidative stress in the...
QSAR Studies on Some C-21 Mercapto Derivatives as AntiPulmonary Inflammatory Agents
Glucocorticoids have been widely used for the treatment of inflammatory conditions but are associated with various side effects. C-21 mercapto derivatives, a class of dissociated steroids, have shown potential as anti-in...
On Autism: One Case
In this paper, we develop an equation for Autism. We make use of detailed knowledge of his diet and behaviour. The basic problem is that he has too much serotonin which enlivens the senses. It’s the opposite of depress...
Effect of Sodium Nitrate (NaNO3) on Sperm Motility and Abnormality: An In vitro Approach
Nitrates (NO3) are the naturally occurring inorganic ions with beneficial effects in both plants and animals. However, NO3 in excess has adverse effect on male and female reproductive systems. The study was undertaken to...