Assessment of the frequency of different Borrelia burgdorferi sensu lato species in patients with Lyme borreliosis from north-east Poland by studying preferential serologic response and DNA isolates
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2013, Vol 20, Issue 1
Abstract
Introduction and objective. Several Borrelia burgdorferi sensu lato species cause Lyme borreliosis throughout Europe and their geographic distribution may influence clinical manifestations of the disease. In Poland, Lyme borreliosis presents mainly with neurologic and cutaneous symptoms, while clinically overt arthritis is rare. The presented study investigates the prevalence of B. burgdorferi s.l. genospecies in a group of patients with different clinical forms and stages of Lyme borreliosis in north-east of Poland. This has not previously been studied.Material and methods. Preferential reactivity towards different B.burgdorferi s.l. species was investigated with a previously validated immunoblot assay in sera of 33 patients with disseminated Lyme borreliosis: 10 with neuroborreliosis, 6 with acrodermatitis chronica atrophicans and 17 with osteoarticular symptoms. Also typed were B.burgdorferi s.l. DNA isolated from the skin and synovial fluid of 7 patients with erythema migrans, acrodermatitis chronic atrophicans and arthritis.Results. Preferential reactivity was detected in 30 out of 33 serum samples. Of these, 25 reacted preferentially with B.afzelii, 3 with B. garinii and 2 with B. burgdorferi ss. B.burgdorferi DNA was isolated from all studied samples and typed as B.afzelii in 5. In a patient with acrodermatitis chronica atrophicans studied with both methods simultaneously, B.afzelii was identified by both genotyping and serotyping.Conclusions. Both methods gave consistent results, indicating B.afzelii as the main agent of all the clinical forms of the Lyme borreliosis in the study area.
Authors and Affiliations
Sambor Grygorczuk, Olivier Peter, Maciej Kondrusik, Anna Moniuszko, Joanna Zajkowska, Justyna Dunaj, Wioletta Żukiewicz-Sobczak, Sławomir Pancewicz
Keywords
Related Articles
- EP ID EP80573
- DOI -
- Views 139
- Downloads 0
Psychosocial conditioning of depressive disorders in post-menopausal women
Studies confirm that the period prior to, during, and some time after menopause are the times of increase risk of the development of depressive disorders. It is indicated that the level of depression at this stage of a w...
Exposure levels of farmers and veterinarians to particulate matter and gases uring operational tasks in pig-fattening houses
The main objective of the study was to assess particulate matter (PM) exposure levels for both the farmer and the veterinarian during different operational tasks in pig-fattening houses, and to estimate their exposure le...
Prevalence and distribution of VRE (vancomycin resistant enterococci) and VSE (vancomycin susceptible enterococci) strains in the breeding environment
[b]Introduction.[/b] Intensive animal production causes numerous problems. Facilities connected with animal maintenance not only cause environmental pollution, but also pose a great sanitary and epidemiological threat. L...
Synthesis of Th17 cytokines in the culture of peripheral blood mononuclear cells stimulated with Borrelia burgdorferi sensu lato
[b]Introduction and objective. [/b]Th17 lymphocytes and their cytokines, interleukin 17A (IL-17A), IL-17F and IL-22, participate in the response to extracellular bacteria and in the autoimmunity and may be engaged in the...
Neurofeedback training for peak performance
[b]aim[/b]. One of the applications of the Neurofeedback methodology is peak performance in sport. The protocols of the neurofeedback are usually based on an assessment of the spectral parameters of spontaneous EEG in re...