Assessment of the Influence of Transgenic Cotton on Beneficial Soil Rhizosphere Microbes
Journal Title: Biotechnology Journal International - Year 2017, Vol 19, Issue 3
Abstract
In this study the impact of Bt cotton has been accessed on the population of beneficial microbes in the rhizosphere of transgenic Bt cotton line (RCH-2 BG II) and its counterpart (a non-Bt cotton line). The Bacillus thuringiensis (Bt) proteins i.e., Cry1Ac and Cry2Ab were released from root exudates of Bt cotton (RCH-2 BG II) was confirmed by qualitative Enzyme-Linked Immunosorbent Assay (ELISA). Rhizosphere soil samples were collected in fifteen days interval i.e., 15, 30, 45 and 60 days after sowing (DAS). Azospirillum brasilense, Bacillus megaterium, Pseudomonas fluorescens population was more on 15th day in non Bt rhizosphere than Bt except Trichoderma viride, where as in 45th and 60th day all four microbes were more in Bt cotton (RCH-2 BG II) rhizosphere than its counterpart RCH-2. The statistical analysis of the data obtained from the pot cuture showed no significant differences in the numbers of CFU of Azospirillum brasilense, Bacillus megaterium (biofertilizer), Pseudomonas fluorescens and Trichoderma viride (biocontrol agents) between rhizosphere soil of Bt and non-Bt cotton. Restriction enzyme analysis of 16S rRNA of Azospirillum brasilense, Bacillus megaterium (biofertilizer), Pseudomonas fluorescens and ITS region of Trichoderma viride obtained from Bt and non Bt cotton rhizosphere in different intervals showed similar banding pattern between them.
Authors and Affiliations
Sivaji Mathivanan, Sridar Raghupathi
Keywords
Related Articles
- EP ID EP315063
- DOI 10.9734/BJI/2017/32592
- Views 153
- Downloads 0
Diabetes Mellitus: Can Stem Cells be the Answer?
This review aims to enlighten the readers regarding the past, present and future of stem cells in the treatment of Diabetes. Diabetes is one of the leading causes of morbidity and mortality, affecting more than 415 milli...
Contents of Research Activities in Immunology Unit of a Tertiary Institution in Ibadan, Southwestern Nigeria
Background: Immunology laboratories carry out researches and training coupled with accurate diagnosis and prognosis of diseases for effective treatment and elimination of diseases. Information on the patterns of research...
Assessment of Roof Harvested Rainwater in Parts of Anambra State for Environmental Pollution Monitoring
Questions have been raised about the quality of roof harvested rainwater which has been shown to be temporally and spatially variable and at times not in compliance with drinking water guidelines. This study is therefore...
In vitro Antibacterial Activity of Organic Extracts from North-West Moroccan Medicinal Plant Myrtus communis (L.)
Aims: This study was designed to evaluate the antibacterial activity of methanol, ethanol, ethyl acetate and n-hexane extract from the leaves of Myrtus communis (L.) growing in North-West of Morocco. Study Design: In vi...
Haematological and Serum Biochemical Indices of Growing Rabbits Fed Diets Containing Varying Levels of Moringa oleifera Leaf Meal
Aims: To determine the influence of Moringa oleifera leaf meal (MOLM) on haematological and serum biochemical indices of growing rabbits fed diets containing varying levels of Moringa oleifera leaf meal. Study Design: Co...