Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth
Journal Title: Journal of Applied Veterinary Sciences - Year 2016, Vol 1, Issue 1
Abstract
This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures; the study proved that the optimum time was 24 hours after subculture of VERO cell line using MOI 2: 1. It was also found that the best time of harvestation of virus fluid of PPR was 9th day post-inoculation to reach the best titre 6 log10 TCID50 /ml.
Authors and Affiliations
El-Dakhly A. T. , Youssef M. M. , Abeer A. Tammam, Namaa A. Mohamed
Keywords
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- EP ID EP677144
- DOI 10.21608/JAVS.2016.62123
- Views 314
- Downloads 1
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