Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats
Journal Title: Journal of Applied Veterinary Sciences - Year 2022, Vol 7, Issue 2
Abstract
Experiments on pulmonary pharmacology are often performed in laboratory animals. Investigators collect organs, samples, or record modifications in the respiratory system during anaesthesia, which might significantly influence the data attained. Indeed, it is recommended to cautiously select the anaesthetic adapted for lung studies. This experiment aimed to evaluate three selected anaesthetics usually used in laboratory animal experiments, namely, (Zolite-rumpon (Z+R) mixture, halothane (HAL), and urethane (URE)) in rats. Rats were divided into four groups, control group, kept without treatment; Zolitel and Rumpon (Z+R) at a dose of 5 and 30 mg/kg body weight intraperitoneally, respectively; HAL group (5% with oxygen supply), and URE (1.5 mg/kg body weight) by inhalation. Blood samples were collected to determine the pH of the blood, bicarbonate (HCO3-), carbon dioxide partial pressure (pCO2), oxygen partial pressure (pO2), alveolar oxygen (A), saturated oxygen (O2 Sat), magnesium (Mg2+), calcium (Ca2+), sodium (Na+), potassium (K+), and chloride (Cl−) ions, haematocrit (Hct), and haemoglobin (Hb). The result indicates that the Z+R mixture causes slight to no differences in the measured data. At the same time, HAL and URE greatly reduced the blood pH and significantly disrupted the values of HCO3-, pCO2, pO2, A, and O2 Sat. Together, HAL and URE induced metabolic acidosis and respiratory depression. Hence, the Z+R mix is suitable for the three anaesthetics pulmonary pharmacology experiments.
Authors and Affiliations
Gareeballah Osman Adam
Keywords
Related Articles
- EP ID EP702598
- DOI 10.21608/JAVS.2022.119861.1123
- Views 83
- Downloads 0
Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen
This study aimed to determine the proper sperm dose per laparoscopic intrauterine insemination (LAI) in Zaraiby goats. Thirty-six Zaraiby goats, two Zaraiby and two Boer bucks were used in the study. Does were divided i...
Detection of Internal Parasites in Turkeys in Erbil city
This study was designed to investigate the prevalence of gastrointestinal parasites in domestic turkeys and infection rates of gastrointestinal parasites infesting turkeys and the relationship with their ages. Seventy-on...
Assessment of Developmental Competence of Holstein Bulls Spermatozoa upon Addition of Aloe Vera Raw Extract during In Vitro Capacitation
The study aimed to evaluate the effect of the addition of aloe vera raw extract on the capacitation of bovine spermatozoa during the in vitro fertilization process. Hyper activated motility (HAM) and acrosome reaction (A...
Impact of Eruca sativa Leaves Aqueous Extract on Liver Function, Immunity Profile and Behavioural Responses of Healthy and Thioacetamide Intoxicated Male Albino Mice
The current experiment was intended to investigate the hepatoprotective, immunostimulant activity, behavioural and anxiety responses of mice supplemented with Eruca sativa aqueous extract (ESAE) at a dose of 1% in drinki...
Protective Effect of Moringa on Salivary Glands of Rats Exposed to Electromagnetic Radiation of Mobile Phone: A Histological Study
The growing widespread usage of cell phones has resulted in potential negative impacts on human health due to their use near the location of salivary glands, which might be affected by non-ionizing radiofrequency radiati...