Assessment of viability responses of refinery effluent bacteria after exposure to phenol stress
Journal Title: Journal of Research in Biology - Year 2011, Vol 1, Issue 8
Abstract
Toxicity of phenol on two bacterial strains isolated from biological treatment unit of petroleum refinery effluent was assessed based on viability responses after exposure to increasing doses of phenol. Viability was based on the reduction of TTC via dehydrogenase activity in viable cells but not by dead cells after 1, 2, 3 and 4 h exposure to phenol. The viability responses of the organisms to phenol are time dependent. At 1, 2 and 3 h of exposure, there was a stimulation of dehydrogenase activity in Pseudomonas sp. RBD1 and Bacillus sp. RBD2. In Pseudomonas sp. RBD1, dehydrogenase activity was progressively inhibited at phenol concentrations greater than 250 mg/l at 1, 2 and 3 h. In Bacillus sp. RBD2, dehydrogenase activity was also progressively inhibited at concentrations greater than 250 mg/l at 1h. Total inhibition occurred in 4 h exposure at all concentrations of phenol in both organisms. The IC50 ranges from 190.605 ± 9.786 to 1070.546 ± 23.127 mg/l. The results of the viability responses assessment of the bacterial strains indicated that the toxicity of phenol depends on the duration of exposure of the organisms to the test chemical
Authors and Affiliations
Nwanyanwu CE, Abu GO
Keywords
Related Articles
- EP ID EP564086
- DOI -
- Views 80
- Downloads 0
Identification lethal and sub lethal concentrations (LC50) of Organophosphate (OP) pesticide Diazinon using an endemic species (Yucatan Molly, Poecilia velifera Regan 1914) as a potential biomonitor for the intensive agricultural activities of Southeastern Mexico
Organophosphate (OP) pesticides are commonly used in agriculture; this group of compounds includes very toxic chemicals. Diazinon (IUPAC name: O,O-Diethyl O-[4-methyl-6-(propan-2-yl)pyrimidin-2-yl] phosphorothioate, INND...
Ambient concentration of suspended particulate matter and manganese in urban area of Madurai City
Air quality in cities is the result of a complex interaction between natural and anthropogenic environmental conditions. Air pollution in cities is a serious environmental problem-especially in the developing countries....
Snakes of the Bhopal district, Madhya Pradesh, India with special reference to road mortality
A checklist of snakes observed in the Bhopal district of Madhya Pradesh from March 2013 to September 2015 is documented here. Seventeen species of the snakes belonging to five families were recorded during the current st...
DNA barcoding, phylogenetic relationships and speciation of Genus: Plectropomus in Andaman coast
DNA barcode technique has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. The present study carried out for the barcode sequences for identifi...
Efficacy of the plant products Acorus calamus, Piper longum, and Econeem against the Larvae of Tobacco Caterpillar Spodoptera litura Fab. (Lepidoptera: Noctuidae)
Larvicidal efficiency of ethanolic extracts of Acorus calamus, Piper longum and Econeem (neem product) were tested against tobacco caterpillar, Spodoptera litura by leaf disc assay method. The results related to Acorus c...