Association of 5’AMP-activated protein kinase activity with disease duration and HbA1c content in leukocytes in diabetic patients
Journal Title: Міжнародний ендокринологічний журнал - Year 2019, Vol 15, Issue 1
Abstract
Background. 5’AMP-activated protein kinase (AMPK) is an enzyme that controls the cell energy balance. With energetic stress in the cell and an increase in the AMP concentration, ATP is replaced by AMP in the exchange centers, resulting in the allosteric activation of AMPK by phosphorylation of 172 threonine within alpha subunit of LKB1 complex in response to changes in cell energy or CAMKKβ, which activates intracellular Ca2+. The purpose was to study the activity of the main energy sensor of cells — AMPK in leukocytes in patients taking insulin preparations, metformin, and other hypoglycemic drugs in association with disease duration and glycated hemoglobin content. Materials and methods. The diabetic patients receiving single-drug or combined therapy with insulin and its analogues, metformin, dapagliflozin and sulfonylureas were randomized into 5 groups: the first group — with an HbA1c level close to the norm — 6.9–7.6 %; the second group — 7.6–9.0 %; the third group — > 9 %; the fourth group > 10 %; the fifth group — > 11 %. To determine the amount of phospho-AMPK (p-Thr172), ELISA kits were used. To get the calibration curve for the AMPK determination, a kidney cell culture HEK293T of the human embryonic kidney was used, which is recommended by manufacturer as a positive control. Results. It was shown that with increase of blood HbA1c, the level of AMPK activity in leukocytes gradually decreased. With increase of blood HbA1c, the level of AMPK activity in leukocytes gradually decreased. The activity of AMPK in leukocytes of patients with HbA1c > 11 % was more than 3.5-fold lower compared to the group with 6.9–7.6 % of HbA1c; AMPK activity in leukocytes in patients with disease duration of 20 years was 3-fold lower. Thus, the AMPK activity in leukocytes may be an indicator of diabetic compensation in diabetic patients. Conclusions. With increase of blood HbA1c, the level of p-AMPK in leukocytes gradually decreased. AMPK activity in leukocytes in diabetes patients with disease duration of 20 years was 3-fold lower than in patients with 10-year experience.
Authors and Affiliations
L. K. Sokolova, V. M. Pushkarev, Yu. B. Belchina, V. V. Pushkarev, T. S. Vatseba, N. D. Tronko
Assessment of Glycemia Impact on the Parameters of Sleep in Type 1 Diabetes Mellitus: the Results of Cross-Sectional Cohort Trial
This study demonstrates the impact of glycemia according to daily monitoring of blood glucose on parameters of nocturnal sleep in type 1 diabetic patients, regardless of the glycated haemoglobin level.
Features of Some Hormonal Parameters in Patients with Perinatal Losses
The presence of mixed urogenital infections during pregnancy leads to violations in mother’s body: changes of homeostasis, depression of the immune response, induction of the autoimmune processes resulting in reduced res...
Results of the Dynamic Cohort Study of Parathyroid Gland Function in Patients with Secondary Hyperparathyroidism
In 92 patients with end-stage chronic kidney disease we have evaluated serum levels of parathyroid hormone (PTH), vitamin D3, indicators of phosphorus and calcium metabolism and markers of bone metabolism: at baseline an...
Morbidity and prevalence of malignant neoplasms of the thyroid gland in Ukraine and Kyiv region after the Chernobyl Nuclear Power Plant accident
Background. The annual increase in the number of newly diagnosed cases of malignant neoplasms of the thyroid gland (MNTG) is observed both in the territories most contaminated with radioiodine after the Chernobyl acciden...
Influence of Endocrine Diseases on Reproductive System Functioning
In the lecture the questions of endocrine diseases influence on the reproductive system functioning are being considered. The reproductive system of woman is analyzed as a complex of interdependent structural elements: h...