Association of Circulating Insulin-like Growth Factors and IGF Binding Protein with Early Cases of Cancer Cervix
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 3
Abstract
ABSTRACT Introduction: Insulin-like Growth Factor (IGF) signaling pathway has important roles in regulating cellular proliferation, apoptosis, differentiation and transformation. IGF-axis is implicated in pathogenesis of several common cancers including cancer cervix and its pre cancer stage. Aim: To measure the circulating levels of IGF-I, IGF-II and IGFBP-3 and evaluate their association with early stages of cancer cervix. Materials and Methods: The present case-control study consisted of 29 histologically proven cases of early cancer cervix (FIGO Stage I and II) and 37 age matched cytologically proven normal healthy controls. The study was conducted in Department of Biochemistry, Maulana Azad Medical College, Delhi, India from May 2004-April 2007. Peripheral blood was drawn in a heparinized vial and plasma was separated by centrifugation and stored at -80°C till analysis. Concentration of IGF-I, IGF-II and IGFBP-3 was measured using commercially available ELISA kit (DRG Diagnostics) in units of ng/mL. Statistical analysis was done using SPSS statistics (V21.0) and Microsoft excel software, viz. Student-t-test for comparison of significant mean, binary logistic regression was used to measure Odds Ratios (OR). Bio-Effective Mitogen (BEM) was calculated as (IGF-I in nM+IGF-II in nM+100)–(90% IGFBP-3 in nM) and mitogenic index as BEM/10% IGFBP-3 in nM. Results: Plasma levels of IGF-I were significantly decreased (p<0.01) while IGF-II levels were not significantly different between cases and controls (p=0.12). IGFBP-3 levels were decreased significantly in cases than controls (p<0.01). Mitogenic index was significantly increased in cases as compared to controls (p=0.01). OR for cancer cervix was calculated based on distribution of 50th percentile in control group and OR (95% CI) was 3.5 (1.04-11.73); p=0.04. Conclusion: Circulating IGF-I, IGF-II and IGFBP-3 are in a dynamic relationship; hence, mitogenic index was educed from these factors. We found mitogenic index to be associated with cancer cervix in concordance to the cognizant role of IGFs and their binding proteins.
Authors and Affiliations
Praveen Sablania, Montosh Chakraborty, Debajit Bagchi
Keywords
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- EP ID EP501667
- DOI 10.7860/JCDR/2018/34888.11333
- Views 37
- Downloads 0
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