Association of G.2686t>C Mutation of Mbl1 Gene With Reproduction Traits in Sahiwal Cattle
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 6
Abstract
Mannose-binding lectin (MBL) is one of the potent constituent of defense system. MBL gene possibly contributes to bacterial infection resistance and was proposed as a molecular marker for reproductive health. Randomly 24 Sahiwal cows were selected to screen for g.2686T>C point mutation in exon 2 of MBL1 gene and its association with Age at First Calving (AFC) and First Calving Interval (FCI). Targeted genomic sequence encompassing targeted g.2686T>C mutation was amplified and digested with Hae III to genotype reported SNP. Genotype analysis using PCRRFLP revealed a polymorphic banding pattern. The preliminary information obtained from the study indicated that the targeted variant of MBL1 gene in indigenous cattle had no association with Age at first calving (AFC) and first calving interval (FCI). However, TT genotype animals revealed lowest AFC (1208.27± 98.35 days) compare to TC (1215±89.85 days) and CC (1261.43±78.30) whereas, TC genotype animals revealed lowest FCI (420.78±79.30) compared to TT (484.33±85.82) and CC (515.21±66.48) genotype animals. Since present study was studied on small sample size, further studies with large sample size are required to explore fully the association of g.2686T>C in sahiwal cattle.
Authors and Affiliations
Kamaldeep . , Ankit Magotra, B. L. Pander, Anika Malik, Asha Rani Garg, D. S. Dalal, B. S. Malik
Keywords
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