Association of MDM2 SNP309 and TP53 Arg72Pro Polymorphisms with Risk of RA in the Algerian Population
Journal Title: Journal of Medical Science And clinical Research - Year 2014, Vol 2, Issue 7
Abstract
This study examines two common, functional, single nucleotide polymorphisms (SNP) in the genes coding the human homolog of murine-double-minute-2 (MDM2) and P53 in patients with rheumatoid arthritis (RA) based on the hypothesis that P53 may be an important negative regulator of the pro-inflammatory transcription factor nuclear factor kappa b (NFkB). Genomic DNA was obtained from 101 patients with RA who fulfilled at least 4 ACR criteria and from 91 healthy controls. Mdm2 SNP309 and p53 P72R were genotyped by polymerase chain reaction and restriction enzyme analysis. In RA patients the frequencies of the p53 P72R, a significant difference in the frequencies of TP53 R72P genotypes or the 72P allele was observed between cases and controls (76.24% of 72 R/P and P/P genotype in cases and 20.88% in controls, p<0.05; 63% of 72P allele in cases and 12.09% in controls, p<0.05). Concerning MDM2 SNP309 G allele, it was associated with an increased risk of RA with OR of 1.80 (95% CI 1.20–2.70), p=0.004. A combined analysis of both polymorphisms revealed a statistically significant association was observed between the increased risk of RA and the combined genotypes of TP53 Arg/Pro +Pro/pro and MDM2 TT ; TP53 Arg/Pro +Pro/pro and MDM2 TG+GG (OR 3.9, 95%CI 1.30- 11.61, p= 0.01) We can conclude that, there is an association of MDM2 SNP 309 G and/or p53 homozygous genotypes Pro/Pro with the risk of developing RA among Algerian patients.
Authors and Affiliations
Fouzia Benrrahal
Keywords
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