Association of Toll-like Receptor 9 (TLR9) Alterations in Malaria Susceptibility and Severity: ATribal Population Based Study in the Malaria Endemic State of Assam, India
Journal Title: Journal of Krishna Institute of Medical Sciences University - Year 2018, Vol 7, Issue 2
Abstract
Background: Toll-like receptors (TLRs) are group of trans-membrane proteins that recognizes specific molecular pattern and activates the downstream cytokines for the efficient clearance of pathogens. Objectives: To evaluate the role of TLR9 1486T/C polymorphism and its association with the malarial pathogenicity and severity. Material and Methods: Present study was a cohort based study and a total number of 150 samples were drawn from three major tribal population group's viz., Karbi, Dimasa and Boro-Kachari. TLR9 (1486T/C) gene polymorphism was assessed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Differential TLR9 m-RNA expression was assessed using Real time PCR (RT-PCR). Results: TLR9 1486T/C genotype variant showed increased risk for the susceptibility of malarial infection (p=0.141) compared to controls. The variant genotype showed significant increased risk with susceptibility to severe malaria infection (p=0.047) compared to uncomplicated malaria (UC-M). TLR9 mRNA level expression showed up-regulation in UC-M compared to controls. Decreased TLR9 expression was found in SM cases compared to UC-M (p=0.312). Significant down-regulation of TLR9 gene was found in UC-M with variant C genotype cases compared to wild types (p= 0.039). Conclusion: Our finding indicates the association of the TLR9 gene polymorphism and linked differential expression modulation in the development of malarial pathogenicity.
Authors and Affiliations
Manash P. Kalita, Subhash Medhi, Sujoy Bose, Dipankar Bujarbaruah, Tarun K. Basumatary, Ramie H. Begum
Keywords
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