ASSOCIATION OF UBE2L3 GENE POLYMORPHISM AND INTERLEUKIN 10 GENE POLYMORPHISM WITH SYSTEMIC LUPUS ERYTHEMATOSUS AMONG WOMEN POPULATION IN CHINA
Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2017, Vol 4, Issue 10
Abstract
Objective: To investigate the association of UBE2L3 gene polymorphism and Interleukin 10 gene polymorphism with systemic lupus erythematous among woman population in china. Methods: A case－control study of 111 female SLE patients and 120 healthy individuals were designed. Genomic DNA of subjects were extracted and amplified by polymerase chain reaction (PCR). second generation/next generation sequencing technology was performed to determine the single nucleotide polymorphism(SNP) in UBE2L3(rs5754217) and IL10(rs3024505). Results: The loci of UBE2L3 rs5754217 show the frequency of genotype GT, GG, TT as follows (52.2% and 44.2%), (18.9% and 30%) and (28.8% and 25.8%) respectively in SLE case group and control group. The frequency of G and T allele as follows (45% and 55%) and (52.1% and 49.7%) respectively in SLE case group and control group. The P values, odds ratio and 95%CI of genotype GT (P=0.10, OR=1.38,95%CI= (0.824 – 2.322)) and genotype TT (P=0.30, OR=1.16,95%CI= (0.651 – 2.075)) are statically not significant(P>0.05). The P values, odds ratio and 95%CI of genotype GG (P=0.02, OR=0.54, 95%CI= (0.294 – 1.006)) are statically significant result (p<0.05). this result indicates that may be there is 0.54 times higher risk for SLE by genotype GG. The P values, odds ratio and 95%CI of allele G (P= 0.06, OR= 0.75, 95%CI = (0.522 – 1.087)) and allele T (P=0.06, OR=1.32, 95%CI = (0.919 – 1.912)) are statically not significant. Loci of IL10rs3024505 show the frequency of genotype CT, CC as follows (7.2% and 11.7%) and (92.8% and 88.3%) respectively in SLE case group and control group. The frequency of C and T allele as follows (96.4 and 94.2%) and (3.6% and 5.8%) respectively in SLE case group and control group. The P values, odds ratio and 95%CI of genotype CT (P=0.12, OR=0.58,95%CI= (0.236 – 1.460)) and genotype CC (P=0.12, OR=1.70,95%CI= (0.690– 4.224)) are statically not significant(P>0.05). The P values, odds ratio and 95%CI of allele C (P= 0.13, OR=1.65, 95%CI = (0.684 – 4.013)) and allele T (P=0.13, OR=0.60,95%CI = (0.249 – 1.461)) are statically not significant. Conclusion: The results suggest that Genotype ―GG‖ of loci rs5754217 (UBE2L3) show a positive correlation with SLE case group as compared to control group. This indicates that the genotype ―GG‖ of rs5754217 may increase the risk of SLE in Chinese women population.
Authors and Affiliations
Song Jiquan
Keywords
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