Assortment of Cold-Active Alkaline Protease-Producing Bacterial Species Isolated From Three Different Lake Samples of Kashmir, India
Journal Title: Journal of Environmental and Social Sciences - Year 2018, Vol 5, Issue 1
Abstract
Extensively used application was applied to achieve cold-active alkaline protease-producing microorganisms for Lake Samples from Kashmir, India. About 100 µl dilutions of soil sample were initially allowed for microbial growth by long term incubation at cold temperatures in nutrient agar medium. Appropriate colonies of bacteria were observed, marked and picked from nutrient agar plates and further screened at different temperatures (5-20 °C) and alkaline pH (6-12) conditions. Amongst 28 potential bacterial isolates, 5 protease producing isolates were optimized for cultural conditions and were found to be producing maximum amount of enzymes in between 15 to 25 °C and pH 9 to 10, classifying the isolates as psychrotrophic and alkalitolerants. The selected 5 isolates belonged to four different genera i.e. Staphylococcus, Serratia, Bacillus and Pseudomonas. The crude enzyme from Bacillus (WLCP1) was further characterized showing maximum protease activity at pH 10 and 15 °C respectively suggesting the biotechnological potential of the isolate.
Authors and Affiliations
Sharma S
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