Atractylenolide Ⅰ protects against lipopolysaccharide-induced disseminated intravascular coagulation by anti-inflammatory and anticoagulation effect
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2017, Vol 10, Issue 6
Abstract
Objective: To investigate whether atractylenolide Ⅰ (ATL-Ⅰ) has protective effect on lipopolysaccharide (LPS)-induced disseminated intravascular coagulation (DIC) in vivo and in vitro, and explore whether NF-kB signaling pathway is involved in ATL-Ⅰ treatment. Methods: New Zealand white rabbits were injected with LPS through marginal ear vein over a period of 6 h at a rate of 600 mg/kg (10 mL/h). Similarly, in the treatment groups, 1.0, 2.0, or 5.0 mg/kg ATL-Ⅰ were given. Both survival rate and organ function were tested, including the level of alanine aminotransferase (ALT), blood urine nitrogen (BUN), and TNF-a were examined by ELISA. Also hemostatic and fibrinolytic parameters in serum were measured. RAW 264.7 macrophage cells were administered with control, LPS, LPS + ATL-Ⅰ and ATL-Ⅰ alone, and TNF-a, phosphorylation (P)-IkBa, phosphorylation (P)-NF-kB (P65) and NF-kB (P65) were determined by Western blot. Results: The administration of LPS resulted in 73.3% mortality rate, and the increase of serum TNF-a, BUN and ALT levels. When ATL-Ⅰ treatment significantly increased the survival rate of LPS-induced DIC model, also improved the function of blood coagulation. And protein analysis indicated that ATL-I remarkably protected liver and renal as decreasing TNF-a expression. In vitro, ATL-I obviously decreased LPS-induced TNF-a production and the expression of P-NF-kB (P65), with the decrease of P-IkBa. Conclusions: ATL-Ⅰ has protective effect on LPS-induced DIC, which can elevate the survival rate, reduce organ damage, improve the function of blood coagulation and suppress TNF-a expression by inhibiting the activation of NF-kB signaling pathway.
Authors and Affiliations
Xi Lin, Liang-Cai Wu
Keywords
Related Articles
- EP ID EP267436
- DOI 10.1016/j.apjtm.2017.06.007
- Views 76
- Downloads 0
Mitochondrial oxidative damage and apoptosis induced by high glucose through Rho kinase signal pathway in renal tubular epithelial cells
Objective: To investigate the role of oxidative stress in human renal tubular epithelial cells (HK-2) induced by high glucose and the underlying signal pathway in vitro. Methods: MYPT1, pro-caspase-3, PGC-1α, and Drp1 pr...
Observational study to assess pregnant women’s knowledge and behaviour related to toxoplasmosis in Essaouira province, Morocco
Objective: To assess knowledge and behaviour related to toxoplasmosis which remains a neglected disease in Morocco. Methods: Observational investigations were conducted among 600 pregnant women from Essaouira Province. T...
Tuberculosis/toxoplasmosis co-infection in Egyptian patients: A reciprocal impact
Objective: To assess the concurrent toxoplasmosis infection in Egyptian TB patients and the impact of each infection on the other in terms of increased severity of TB or reactivation of latent Toxoplasma infection. Metho...
Molecular characterization and subtyping of Blastocystis in urticarial patients in Turkey
Objective: To investigate Blastocystis’ etiologic role and association with gastrointestinal symptomatology in acute and chronic urticaria patients and to identify Blastocystis subtypes responsible for urticaria. Methods...
Novel PCR primers to diagnose visceral leishmaniasis using peripheral blood, spleen or bone marrow aspirates
Objective: To establish a suitable method of diagnosis of visceral leishmaniasis (VL) using peripheral blood, spleen or bone marrow aspirates. Methods: Peripheral blood, bone marrow and spleen aspirate samples were colle...