Autologous Platelet Rich Plasma Injection Improves Early Tendon Repair in Rabbits: A Histopathological and Biomechanical Study
Journal Title: Iranian Journal of Veterinary Surgery - Year 2017, Vol 12, Issue 1
Abstract
Objective- The aim of this study was to investigate the PRP effects on the early time-period during tendon healing in rabbits DDF tendon. Design-Experimental study Animals- Twenty male New Zealand white rabbits Procedure-PRP samples were prepared using twice centrifugation method of modification of the Cuarsan technique. Animals were randomly assigned into two equal treatment and control groups. The injury model was unilateral complete transection through the middle one third of deep digital flexor tendon. Immediately after primary repair, either 0.5 cc PRP or placebo was injected intratendiously into the suture site in the treatment and control groups, respectively. Operated limbs were immobilized for two weeks. Animals were sacrificed at the third week and the tendons underwent histopathological (H&E and MT staining) and biomechanical evaluation. Results- The histopathological (H&E) observation showed significant increase in percentage of fibrillar linearity, fibrillar contiuity, number of capillaries in epitenon and epitenon thickness in PRP treated group compared to the control group (p<0.05). Results from MT staining revealed more collagen deposition, in treatment group in comparison to the control. Results of mechanical testing revealed the significant improvement of force at failure and energy absorption capacity of repaired in the PRP group in comparison to the control (p<0.05). Conclusion and clinical relevance-The present study findings suggest that PRP is a simple, safe, quick and cost effective way to obtain a natural concentration of autologous growth factors which reduce the risk of rupture after tendon primary repair and improve functional outcomes.
Authors and Affiliations
Sara Javanmardi, Amirata Vosough, Raziallah Jafari Jozani, Javad Ashrafi-Helan
Keywords
Related Articles
- EP ID EP633109
- DOI 10.22034/ivsa.2017.50348
- Views 167
- Downloads 0
Clinicopathological Features of Contagious Ecthyma-Like Lesions in a Dog
Background: Contagious ecthyma or orf is a poxviral disease of goat and sheep, with incidental infection appearing in humans, cows, camels, and many wild ruminants, and very rarely in dogs. Case description: In the prese...
Evaluation of Mastitis Impact on Lameness and Digital Lesions in Dairy Cows
Objective: This current study was done to find any correlation between clinical mastitis and lameness occurrence and incidence in dairy farms. Design: This prospective field trial was done on a case control study basis....
The Protective Effect of Orally Administered Amlodipine against Intestinal Ischemia-Reperfusion Injury in Rats
Objective- This study investigated the effect of amlodipine on intestinal ischemia-reperfusion injury in rats Design-Experimental study Animals-Fifteen male Sprague-Dawly rats weighing 200-220g Procedure- Rats were rando...
In Vitro Evaluation of Equine Fibroblast-Like Synoviocytes Viability Treated with Doxycycline
Objective- The purpose of present study was to investigate the viability of equine fibroblast-like synoviocytes (FLSs) treated with doxycycline. Design- Experimental study. Sample population- FLSs from metacarpophalangea...
Computed Tomographic Anatomy and Topography of the Lower Respiratory System of the Southern White-Breasted Hedgehog (Erinaceus concolor)
Objective- The aim of this study was preparing detailed anatomic images of the thoracic cavity of the southern white-breasted hedgehog using the non-invasive computed tomography (CT) technique. Design- Descriptive study....