Automated Corrected Reticulocyte Count Superiority above Manual Methods
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2016, Vol 4, Issue 4
Abstract
Abstract: Corrected Reticulocyte Count is necessary because the raw reticulocyte count is misleading in anemic patients. The problem arises because the reticulocyte count is not really a count but rather a percentage: it reports the number of reticulocytes as a percentage of the number of red blood cells. In anemia, the patient's red blood cells are depleted, creating an erroneously elevated reticulocyte count. 30 adult male and female anemic patients were randomly selected from the Arth Diagnostics laboratory, Rajasthan. Automated CRC was performed through Pentra XLR from Horiba Medicals, Japan. Manual reticulocyte count was performed traditional method with Methylene Blue dye and light microscopy. Manual PCV was performed through graduated tube centrifuging at high speed. There is significant difference in the values of CRC in manual vs automated method. The variation is huge and it varied from minimum 2.2% to 211%. The average percentage deviation is 34.1%. The p value of difference between Manual CRC and Automated CRC is significant (p value <0.1). The difference was not skewed in one direction rather it was on either side i.e. it varied from positive to negative both significantly. Automated methods are counting through measurement error free methods along with more number of cells in comparison to manual methods. So undoubtedly Pentra XLR from Horiba Medicals, Japan is superior and reliable for estimating Corrected Reticulocyte Count. It will of definite help for better diagnosis and management of anemic patients. Keywords: Reticulocyte, anemia, Pentra XLR, Methylene Blue, light microscope
Authors and Affiliations
Shubham Rastogi, Dr. Arvinder Singh, Dr. Pooja Chhabra
Keywords
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