Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer Cells
Journal Title: Journal of Urological Surgery - Year 2021, Vol 8, Issue 1
Abstract
Objective: Most patients with non-muscle-invasive bladder cancer (NMIBC) do not respond to intravesical Bacillus Calmette-Guerin (BCG) immunotherapy and have high risk of NMIBC recurrence and progression. In addition to its therapeutic effect which increases the local immune response, BCG also exerts an anti-tumour effect by increasing oxidative stress, and producing reactive oxygen species and oxidative DNA damage in bladder cancer (BC) cells. The oxidative DNA damage is repaired by base excision repair (BER) mechanism. Thus, BER capacity of BC cells could be an important factor in response to BCG therapy. Effects of BCG on the activity of BER in BC transitional carcinoma cell line, T24 have been investigated. Materials and Methods: The uracil-initiated total BER and BER enzyme activities were measured in whole cell extracts with or without BCG treatment using a [γ-32P] adenosine triphosphate-labelled 51-mer DNA substrates. Results: BCG treatment increased the activities of uracil-initiated total BER and BER enzymes, uracil DNA glycosylase and DNA polymerase β in 6 h and 24 h repair periods and increased the activity of 8-oxoguanine DNA glycosylase in 6 h repair in T24 BC cell line. Conclusion: The enhanced BER activity in BC cells in response to BCG treatment could be an important factor in BCG resistance.
Authors and Affiliations
Selçuk Keskin, Berna Somuncu, Meltem Müftüoğlu
Keywords
Related Articles
- EP ID EP693407
- DOI 10.4274/jus.galenos.2020.0013
- Views 135
- Downloads 0
Re: Incidences and Oncological Outcomes of Urothelial Carcinoma in Kidney Transplant Recipients
no summary
Fournier’s Gangrene: Analysis of Risk Factors Affecting Mortality in a Tertiary Urology Referral Center
Objective: We aimed to investigate the risk factors affecting mortality in patients with Fournier’s gangrene (FG). Materials and Methods: Records of 48 male patients, who were admitted to our urology clinic between Nove...
Kidney Stone Treatment in the Anomalous Kidney with Retrograde Intrarenal Surgery: A Matched Pair Analysis
Objective: To show our retrograde intrarenal surgery (RIRS) experience in anomalous kidney stones and compare its safety and efficiency with normal kidney stones. Materials and Methods: Between 2012 and 2018, patient da...
Incidental Prostate Adenocarcinoma in Prostate Transurethral Resections: Our Eight Year Experience
Objective: Incidental early-stage prostate cancer (PC) can be detected in patients undergoing transurethral resection of the prostate (TURP) due to benign prostatic hyperplasia (BPH). In our study, we investigated the in...
Comparison of Stone Scoring Systems in Predicting Outcomes of Percutaneous Nephrolithotomy in Patients with Solitary Kidney
Objective: To assess efficacy of the Guy’s Stone score (GSS) and Clinical Research Office of the Endourological Society (CROES) nomogram in predicting outcomes of percutaneous nephrolithotomy (PCNL) in patients with soli...