Bacterial Alkaline Laccase and Its Environmental Applications
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
Alkaline laccase play an important role in bioremediation such as degradation of phenols, aromatic hydrocarbons, and different dyes used in textile industry. Also in denim washing, cosmetics and pulping industry. The isolates from soil sample near the vicinity of wood industry and were screened for alkaline laccase production. The darkness of brown colour of the colony due to oxidation of guaiacol was considered for efficient production. For potent isolate the significant enzyme activity was calculated using ABTS as substrate. The potent isolate was identified by 16s rRNA sequence analysis and found to be Bacillus subtilis. Optimization of incubation period, pH and temperature conditions for enzyme production were determined and found to be 72hrs, pH – 9 and 300C respectively. Optimization of carbon sources for enzyme production were determined and found to be 1% glucose and 1% wheat bran. Optimization of organic nitrogen sources showed maximum enzyme activity at 0.8 % peptone and tryptone. In case of inorganic nitrogen sources the activity was optimum for 2% of ammonium nitrate and ammonium dihydrogen phosphate. The partially purified preparation of enzyme was immobilized and optimized for sodium alginate concentration, bead size. The maximum activity was found at 2%, and 2mm. The enzyme activity of purified enzyme was found 18.8 U/ml. The immobilized beads were recycled up to four cycles for decolourization of textile waste and showed active decolourization up to third cycle. The enzyme was efficient in degrading phenol, model dyes like reactive black and textile effluent.
Authors and Affiliations
JuileePankaj Dhokane, Bholay A. D, Nalawade P. M
Keywords
Related Articles
- EP ID EP391879
- DOI 10.9790/3008-1205070109
- Views 90
- Downloads 0
Assessment of Serum Galectin-8 in Iraqi Patients with Rheumatoid arthritis
Background:Rheumatoid arthritis (RA) is chronic inflammatory arthritis disease with significant morbidity and mortality .Early diagnosis is important for better treatment and outcome.Galectins are potent immune regulator...
“Study And Assessment Of Drug-Drug Interactions In Hospitalised Patients In Quaternary Care Hospital”
Drug-Drug Interactions (DDI) in patients receiving multi-drug therapy are of wide spread. Such interactions are one of the important cause of adverse drug reactions (ADRs) and may lead to increased risk of mortality and...
Drug Control in Public Health Center through the Analysis of ABC Indexes Critical Drugs
Background: The use of drug in Indonesia reached 39% of the cost of healthcare, which many variations of drugs causes its utilization was inefficient. The study aimed to determine the critical index of drugs through anal...
Screening for the protective efficacy of two different extracts of Pithecellobium dulce in CCl4 and Isoniazid induced hepatic injury in wistar rats.
Hepatoprotective effect of ethanolic and hexane:benzene:acetone (1:1:1) extracts of Pithecellobium dulce (EEPD and HBAPD respectively) in carbon tetra chloride (CCl4) and Isoniazid (INH) induced toxicity in wistar rats w...
A Potent Cocktail of Clotrimazole, Ciclopiroxolamine, Zinc Ion and Tridecyl Salycilate Is Capable To Struggle Radically the Pityriasis Versicolor, Even the Repetitive and the Intermittent Ones
The term pityriasis, evoked by Malassezia spp. a yeast, is used to describe skin conditions in which the scale appears similar to bran. The multiple colours of pityriasis versicolor give rise to the second part of the na...