Bacterial Alkaline Laccase and Its Environmental Applications
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2017, Vol 12, Issue 5
Abstract
Alkaline laccase play an important role in bioremediation such as degradation of phenols, aromatic hydrocarbons, and different dyes used in textile industry. Also in denim washing, cosmetics and pulping industry. The isolates from soil sample near the vicinity of wood industry and were screened for alkaline laccase production. The darkness of brown colour of the colony due to oxidation of guaiacol was considered for efficient production. For potent isolate the significant enzyme activity was calculated using ABTS as substrate. The potent isolate was identified by 16s rRNA sequence analysis and found to be Bacillus subtilis. Optimization of incubation period, pH and temperature conditions for enzyme production were determined and found to be 72hrs, pH – 9 and 300C respectively. Optimization of carbon sources for enzyme production were determined and found to be 1% glucose and 1% wheat bran. Optimization of organic nitrogen sources showed maximum enzyme activity at 0.8 % peptone and tryptone. In case of inorganic nitrogen sources the activity was optimum for 2% of ammonium nitrate and ammonium dihydrogen phosphate. The partially purified preparation of enzyme was immobilized and optimized for sodium alginate concentration, bead size. The maximum activity was found at 2%, and 2mm. The enzyme activity of purified enzyme was found 18.8 U/ml. The immobilized beads were recycled up to four cycles for decolourization of textile waste and showed active decolourization up to third cycle. The enzyme was efficient in degrading phenol, model dyes like reactive black and textile effluent.
Authors and Affiliations
JuileePankaj Dhokane, Bholay A. D, Nalawade P. M
Keywords
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- EP ID EP391879
- DOI 10.9790/3008-1205070109
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