BACTERIAL 𝛂-AMYLASE ACTIVITY ON THE STARCH PRESENT IN MAIZE KERNEL
Journal Title: Innoriginal: International Journal Of Sciences - Year 2017, Vol 4, Issue 4
Abstract
Introduction: The purpose of the present research work was to investigate the feasibility of production of alpha amylase enzyme from three bacterial species Bacillus subtilis, Bacillus licheniformis and Lactobacillus acidophilus and to check their applicability on maize starch hydrolysis. The present study was focused on the alpha amylase production from three bacterial species and comparative study of them to hydrolyse the maize starch. Method: For the growth of bacteria and amylase enzyme production from them, they were grown in a nutrient medium. These bacteria can produce alpha amylase enzyme in a nutrient medium. This amylase is responsible for starch hydrolysis, through this study we can find the alternative mean for the starch digestion. As with the age Alpha, amylase production becomes reduced in the human body. The enzyme alpha amylase is required to digest the starch. So, scarcity of this enzyme in the body may cause many diseases associated with carbohydrate, like diabetes and colon cancer. So, when the metabolic rate becomes down, and alpha amylase production is not properly achieved than to digest the food starch in our body we can give this extracted alpha amylase from bacteria in some form. Amylase activity was checked by the Iodine assay and DNSA assay method. Result: Bacteria Bacillus subtilis has shown the highest 𝛂-amylase activity on the starch present in Maize kernel.
Authors and Affiliations
PRIYA VYAS, MRUNAL SHIRSAT
Keywords
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