Bacterial Diversity and Composition in Oylat Cave (Turkey) with Combined Sanger/Pyrosequencing Approach
Journal Title: Polish Journal of Microbiology - Year 2016, Vol 65, Issue 1
Abstract
The microbiology of caves is an important topic for better understanding subsurface biosphere diversity. The diversity and taxonomic composition of bacterial communities associated with cave walls of the Oylat Cave was studied first time by molecular cloning based on Sanger/pyrosequencing approach. Results showed an average of 1,822 operational taxonomic units per sle. Clones analyzed from Oylat Cave were found to belong to 10 common phyla within the domain Bacteria. [i]Proteobacteria[/i] dominated the phyla, followed by [i]Actinobacteria[/i], [i]Acidobacteria[/i] and [i]Nitrospirae[/i]. Shannon diversity index was between to 3.76 and 5.35. The robust analysis conducted for this study demonstrated high bacterial diversity on cave rock wall surfaces.
Authors and Affiliations
Yasemin Gulecal-Pektas
Keywords
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