Bacteriophages specific for DH5α strain of E. Coli from wastewater isolation techniques
Journal Title: Revista MVZ Cordoba - Year 2012, Vol 17, Issue 1
Abstract
Objetive. To develop an isolation technique for DH5α E. coli specific bacteriophages in wastewater. Materials and methods. The 1601 method of the Environmental Protection Agency of the United States of America and an wastewater enterovirus obtaining method were used as a reference. During the development of the protocol, multiple tests were performed using the T4 bacteriophage as positive control and the bacteriophage isolates obtained from wastewater. Results. Plaque-forming units (PFU) were observed; tittering of bacteriophages present in each of the DH5α E. coli cultures was obtained; the relationships between the quantification of PFU in the control and experimental groups, and their corresponding PFU characteristics were determined for each of the experiments. Conclusions. It was possible to establish a microbiological protocol for the isolation of bacteriophages specific for DH5α E. coli.
Authors and Affiliations
Gabriel Gaviria A, Biól| Universidad del Quindío. Facultad Ciencias de la Salud. Centro de Investigaciones Biomédicas. Grupo de Inmunología Molecular (GYMOL). Carrera 15 Calle 12 Norte, Teléfono: +57(6) 7460168. Armenia, Colombia.Correspondencia: jhoncarlos@uniquindio.edu.co, María González de S, M. Sc| Universidad del Quindío. Facultad Ciencias de la Salud. Centro de Investigaciones Biomédicas. Grupo de Inmunología Molecular (GYMOL). Carrera 15 Calle 12 Norte, Teléfono: +57(6) 7460168. Armenia, Colombia., Jhon Castaño O, Ph. D *| Universidad del Quindío. Facultad Ciencias de la Salud. Centro de Investigaciones Biomédicas. Grupo de Inmunología Molecular (GYMOL). Carrera 15 Calle 12 Norte, Teléfono: +57(6) 7460168. Armenia, Colombia.
Frequency of antibodies anti-Neospora caninum in sheeps from the department of Cordoba, Colombia
Objective. To evaluate the frequency of anti-N. Caninum antibodies in two sheep herds in the department of Córdoba, Colombia. Materials and methods. The study was performed in the department of Córdoba (Colombia) in two...
The use of conantokin and polyclonal antibodies to identify the NR2B subunit of the n-methyl-d-aspartate receptor
Objective. To propose a methodology that identifies the NR2B subunit through the use of conantokin G, as well as an adequate extraction of the NR2B subunit. Materials and methods. Two methodologies were tested for the ex...
Postpartum haemoglobinuria in dairy cows of the Bio-Bio region, Chile
Bovine postpartum hemoglobinuria is a metabolic disorder observed sporadically in dairy cows up to 6 weeks postpartum associated to increasing milk production. It is characterized by an acute intravascular hemolytic anem...
Intake, digestibility and intake behaviour in cattle fed different levels of palm kernel cake
The potential use of palm kernel cake was evaluated as a replacement for soybean and corn meal in cattle feed, by investigating their intake, digestibility levels and the intake behaviour of cattle fed diets containing d...
Pulmonary adenocarcinoma in cattle
The Macroscopic, histological and immunohistochemical aspects of lung acinar adenocarcinoma and the presence of nodules in the abdominal cavity of an adult female bovine are reported. In the necropsy analysis samples wer...