Bacteriophages specific for DH5α strain of E. Coli from wastewater isolation techniques
Journal Title: Revista MVZ Cordoba - Year 2012, Vol 17, Issue 1
Abstract
Objetive. To develop an isolation technique for DH5α E. coli specific bacteriophages in wastewater. Materials and methods. The 1601 method of the Environmental Protection Agency of the United States of America and an wastewater enterovirus obtaining method were used as a reference. During the development of the protocol, multiple tests were performed using the T4 bacteriophage as positive control and the bacteriophage isolates obtained from wastewater. Results. Plaque-forming units (PFU) were observed; tittering of bacteriophages present in each of the DH5α E. coli cultures was obtained; the relationships between the quantification of PFU in the control and experimental groups, and their corresponding PFU characteristics were determined for each of the experiments. Conclusions. It was possible to establish a microbiological protocol for the isolation of bacteriophages specific for DH5α E. coli.
Authors and Affiliations
Gabriel Gaviria A, Biól| Universidad del Quindío. Facultad Ciencias de la Salud. Centro de Investigaciones Biomédicas. Grupo de Inmunología Molecular (GYMOL). Carrera 15 Calle 12 Norte, Teléfono: +57(6) 7460168. Armenia, Colombia.Correspondencia: jhoncarlos@uniquindio.edu.co, María González de S, M. Sc| Universidad del Quindío. Facultad Ciencias de la Salud. Centro de Investigaciones Biomédicas. Grupo de Inmunología Molecular (GYMOL). Carrera 15 Calle 12 Norte, Teléfono: +57(6) 7460168. Armenia, Colombia., Jhon Castaño O, Ph. D *| Universidad del Quindío. Facultad Ciencias de la Salud. Centro de Investigaciones Biomédicas. Grupo de Inmunología Molecular (GYMOL). Carrera 15 Calle 12 Norte, Teléfono: +57(6) 7460168. Armenia, Colombia.
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