[b]Direct Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Solanum Nigrum L. A Medicinal Herb[/b]
Journal Title: Journal of Ornamental and Horticultural Plants - Year 2012, Vol 2, Issue 2
Abstract
In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0 – 5.0 mg/l) and KIN (1.0 – 5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple shoots was obtained on MS medium supplemented with BAP (1.0 mg/l) from shoot tip (20.4 ± 0.22) and MS medium supplemented with BAP (3.0 mg/l) from nodal explants (8.4 ± 0.22). The in vitro regenerated shoots were rooted (8.4 ± 0.16 roots per shoot) on MS medium supplemented with NAA (1.0 mg/l) within 2-3 weeks of culture and the regenerated plantlets could be successfully established in soil where they grow normally.
Authors and Affiliations
M. S. Kavitha, E. G. Wesely, P. Mehalingam
Keywords
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