Behavior of different rice explants (Variety IACuba-28) in genetic transformation by Agrobacterium tumefaciens
Journal Title: Biotecnología Vegetal - Year 2003, Vol 3, Issue 1
Abstract
ACT The present work describes the behavior of four rice explants: coleoptiles, embryogenic calli, earliest calli and mature embryos, in genetic transformation via Agrobacterium tumefaciens, applying the protocol established for cuban rice cultivar in our laboratory. We used GUS activity as a signal for measuring transformation efficiency. The best results were obtained with embryogenic calli, in which transformation efficiency was 37 %, and an average of 11.20 blue spots per transformed calli was obtained. In coleoptiles and earliest calli was detected ß-Glucuronidase expression, but it was not detected in mature embryos, whose tissues were affected seriously by bacterial infection. We demonstrated the versatility of this transformation procedure in different tissues of IACuba-28 rice cultivar.
Authors and Affiliations
Maylin Pérez, Daymi Abreu, Annerys González, Julio Alfonso, Raúl Armas
Keywords
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