Behavioral and Histological Observations after the Human Amniotic Epithelial Cells Transplantation in the 6-Hydroxydopamine Induced Parkinsonism Disease Model in Wistar Albino Rats
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2014, Vol 10, Issue 2
Abstract
Several toxin-induced animals’ models simulate the motor deficits occurring in PD. Among them, the unilateral 6-hydroxydopamine (6-OHDA) model is frequently used in rats and has the advantage of presenting side-biased motor impairments. The studies on impairment and improvement of the motor and sensory motor behaviors after the retrograde degeneration with the HAE cells implantation are less or unavailable. In the present work we have studied the amelioration of motor and sensory motor behaviors after the implantation of human amniotic epithelial (HAE) cells in 6-hydroxydopamine (6-OHDA) lesioned rodent model of PD induced rats. The Sterotaxic injection of saline alone (without 6-OHDA) in the striatum could not produce any detectable effects on the behaviors and histological observations. This study demonstrates that substantial and stable improvement of behaviors after the HAE transplantation in unilateral 6-OHDA-induced lesions can be established in rodent model of PD disease, and that these canbe functionally assessed using several different behavioral tests. This would suggest that the HAE cells may be a suitable donor tissue to alleviate various degenerative diseases in animal model before the clinical trial in human who are suffering from the various degenerative diseases. The present study indicates that the transplantation of HAE cells could be a viable therapeutic strategy to slow down the progressive degeneration of striatal DA neurons in the animal model of PD. Thus the neurological degenerative disease can be treated by the grafting the HAE cells and can be attempted for faster and sustained recovery of functional loss in the human clinical trials.
Authors and Affiliations
Ravisankar Periyasamy , Omprakash Kasaragod Venkatkrishnayya , Muthusamy Rathinasamy , Rameshkumar Radhakrishnan , Ravindran Rajan
Keywords
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