Beta-Globin Gene Cluster Haplotypes in Iranian Sickle Cell Patients: Relation to Some Hematologic
Journal Title: Iranian Journal of Blood and Cancer - Year 2012, Vol 4, Issue 3
Abstract
Background: Sickle cell anemia is relatively common in Khuzestan province located in Southwest Iran. The characteristics of sickle cell disease in Iran are apparently different from other regions some of these characteristics might be related to β-chain haplotypes. The purpose of this study was to determine the frequency of β-chain haplotypes in 50 patients with homozygous sickle cell anemia in Khuzestan Province. Materials and Methods: The haplotypes were explored around and within the ε–Gγ–Aγ–ѱβ–δ–β globin gene complex by analysing seven polymorphic restriction sites [(1) HincII 5ʹ to ε (2) XmnI-5ʹγG, (3) Hind III-γG, (4) Hind III-γA, (5) HincII- ѱβ, (6) HincII- 3ʹto Ψβ, and (7) HinfI and RsaI - 5ʹto β], followed by restriction digestion and agarose gel electrophoresis. The effect of beta globin haplotypes upon hematologic parameters such as hemoglobin, hemoglobin F, mean corpuscular hemoglobin concentration, reticulocyte count, serum bilirubin and Lactate Dehydrogenase was also studied. Results: The Arab/Indian was the most frequent haplotype, present in 38 percent of chromosomes, followed by Benin haplotype (18%), Senegal haplotype (16%), Bantu haplotype (16%) and Cameroon haplotype (12%). The mean percentage of hemoglobin F in sickle cell anemia patients was 17.18±8.81%, and in the homozygous Arab/Indian haplotype it was higher (20.90%), but the difference was not significant. The hemoglobin F was significantly higher in females compared to males (19.10 versus 14.50, P<0.005). Conclusion: There was no significant correlation between haplotypes and hematological characteristics like fetal hemoglobin level among our patients.
Authors and Affiliations
B. Keikhaei, H. Galehdari, M Pedram, K Jaseb, SH. Bashirpour, KH. Zandian
Keywords
Related Articles
- EP ID EP368579
- DOI -
- Views 100
- Downloads 0
Therapeutic Plasma Exchange in Pediatric Severe Immune Thrombocytopenia: A Case Report and Literature Review
Treatment of severe Immune Thrombocytopenic purpura (ITP) accompanied by life-threatening bleeding events is challenging and a combination of treatment measures should be undertaken to rapidly increase the platelet count...
FMS-like Tyrosine Kinase-3 Mutation in a Child with Standard-risk ALL and Normal Karyotype
FMS-like tyrosine kinase-3 is a receptor tyrosine kinase expressed by immature hematopoietic cells and is important for the normal development of stem cells and the immune system. Mutations of FMS-like tyrosine kinase-3...
Comparison of Cardiac Function in Young Patients with Thalassemia Intermedia and Healthy Individuals Using Echocardiography Method
Background: Cardiac dysfunction due to chronic anemia and hemosiderosis are the major causes of death among patients with thalassemia intermedia. This study was performed to compare the cardiac function in thalassemia in...
The Role of Adiponectin in Prostate Cancer: A Narrative Review
Prostate cancer (PCa) is the most common type of cancer among men over 60 years old. The aggressiveness and mortality of PCa can be correlated with obesity. Adipose tissue-derived cytokines such as adiponectin may explai...
ABO blood groups and prognosis of breast cancer: a case-control study in Arak/Iran
Background: To explore a possible rela!onship among blood groups and the prognosis of breast cancer, this case- control study was carried out in Arak city, Arak province, Iran. Materials and Methods: One hundred and thir...