Bifidobacterium Longum as an Orally Administered Carrier of LL-37 to Treat Bacterial Diarrhea
Journal Title: Journal of Pharmaceutical and Biomedical Sciences - Year 2017, Vol 7, Issue 6
Abstract
LL-37 is the only antibacterial peptide of the cathelicidin family found in the human body. It has antimicrobial activity and is able to neutralize endotoxins, with no obvious toxic effects on probiotics. In the present study, we engineered Bifidobacterium longum (B. longum) as an experimentally constructed delivery system of LL-37 to treat bacterial diarrhea. The results showed that the engineered B. longum successfully secreted LL-37, at a level of 57 ± 6.8 μg/ml after fermentation for 42 h. The supernatant containing recombinant LL-37 significantly inhibited the growth of Escherichia coli (E. coli ) and Staphylococcus aureus (S. aureus), and markedly decreased TNF-α levels in LPS-induced RAW264.7 cells. This modified B. longum was orally administered after bacterial diarrhea was induced with E. coli, and resulted in significant reduction of the proinflammatory cytokine TNF-α. The anti-inflammatory cytokine IL-10 and mucosal repair factor TGF-α were remarkably increased, and the intestinal bacteria Lactobacillus and Bifidobacterium, as well as E. coli, were well-regulated. Histological observations showed colonic protection with dose-modified B. longum. The results demonstrated effective resistance to bacterial diarrhea with LL-37–secreting B. longum.
Authors and Affiliations
Hanxiao Sun, Qian Zhang, Qing Guo, Shiyu Li
Keywords
Related Articles
- EP ID EP320523
- DOI -
- Views 110
- Downloads 0
Detection of antibacterial activity of Querus infectoria against MRSA isolates
Abstract: Background:- Methicillin-resistant Staphylococcus aureus (MRSA) has been well-documented as a major cause of hospital infections. The aim of the present work was to evaluate the antibacterial potential of aqueo...
Bufadienolides with cytotoxic activity from the eggs of Bufo bufo gargarizans
Seven bufadienolides (1–7) were isolated from the eggs of toad Bufo bufo gargarizans. Their chemical structures were identified on the basis of various spectroscopic methods, including NMR and HRESIMS. These compounds we...
Role of Pathological Organisms in Gallstone Disease
Background Gallstone disease is the commonest disease in India. Patients present with pain abdomen and nausea and vomiting. This study deals with etiology, pathogenesis including the role of bacteria in the formation of...
Venous Thromboembolism Risk and Prophylaxis in Hospitalized Patients in Iraq
Venous thromboembolism (VTE) is a common unrecognized and underestimated preventable condition; there are no data estimating the prevalence of VTE risk factors among the Iraqi patients or information’s about the Iraqi ph...
Pathogenesis of Diabetes and Periodontal Health–a Two-way Connection
Periodontitis has been traditionally regarded as a chronic inflammatory oral infection. However, recent studies indicate that this oral disease may have profound effects on systemic health. Periodontitis is an infection...