Bimolecular identification of M.ovinpeumoniae from nasal swab samples of sheep from various districts of Balochistan, Pakistan
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2016, Vol 4, Issue 1
Abstract
Mycoplasma ovipneumoniae is an important respiratory pathogen of sheep across the world. In the present study nasal swab samples (n=778) were processed and 51isolates of M. ovipneumoniae were identified through biochemical tests and PCR. Difference in the isolation rate among districts (n=12), age groups (n=3), breads (n=6) was assessed. Similarly the isolate rate among healthy (n=513) and respiratory distressed (n=265) sheep were also compared. The results for the district-wise isolation of Mycoplasma ovipneumoniae in sheep showed nonsignificant variations among districts of Balochistan (ï£2= 1.65, P=0.9990). Statistically significant difference (ï£2 = 7.698, P< 0.02) in the isolation of Mycoplasma ovipneumoniae among various age groups was recorded, while highest isolation was seen in sheep of < 1 year.Statistically highly significant difference (ï£2 = 16.6, P <0.0050) in the isolation of Mycoplasma ovipneumoniae among various breeds of sheep in Balochistan was seen, whereas highest isolation rate (3.70%) was observed in Harnai breed for sheep Furthermore, statistically highly significant difference (Fisher's exact test P≤0.0001) in the isolation of Mycoplasma ovipneumoniae between healthy and respiratory distressed sheep breeds in Balochistan was found.
Authors and Affiliations
Shahid Amin| Institute of Microbiology University of Agriculture, Faisalbad, Pakitan, Department of Clinical Medicine and Surgery, University of Agriculture, Faisalbad, Pakitan, Sajjad ur Rahman| Institute of Microbiology University of Agriculture, Faisalbad, Pakitan, Department of Clinical Medicine and Surgery, University of Agriculture, Faisalbad, Pakitan, Iftikhar Hussain| Institute of Microbiology University of Agriculture, Faisalbad, Pakitan, Department of Clinical Medicine and Surgery, University of Agriculture, Faisalbad, Pakitan, Ghulam Muhammad| Institute of Microbiology University of Agriculture, Faisalbad, Pakitan, Department of Clinical Medicine and Surgery, University of Agriculture, Faisalbad, Pakitan, Mohammad Arif Awan| Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistan, Zafer Ahmad| Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistan, Mohammad Masood Tariq| Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistan, Muhammad Irfan Ullah| Department of Clinical Sciences Bahauddin Zakariya University Multan, Pakistan, Muhammad Azam Kakar| Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistan
Keywords
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- EP ID EP13526
- DOI 10.18869/IJABBR.2016.32
- Views 343
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