Bimolecular identification of M.ovinpeumoniae from nasal swab samples of sheep from various districts of Balochistan, Pakistan

Abstract

Mycoplasma ovipneumoniae is an important respiratory pathogen of sheep across the world. In the present study nasal swab samples (n=778) were processed and 51isolates of M. ovipneumoniae were identified through biochemical tests and PCR. Difference in the isolation rate among districts (n=12), age groups (n=3), breads (n=6) was assessed. Similarly the isolate rate among healthy (n=513) and respiratory distressed (n=265) sheep were also compared. The results for the district-wise isolation of Mycoplasma ovipneumoniae in sheep showed nonsignificant variations among districts of Balochistan (2= 1.65, P=0.9990). Statistically significant difference (2 = 7.698, P< 0.02) in the isolation of Mycoplasma ovipneumoniae among various age groups was recorded, while highest isolation was seen in sheep of < 1 year.Statistically highly significant difference (2 = 16.6, P <0.0050) in the isolation of Mycoplasma ovipneumoniae among various breeds of sheep in Balochistan was seen, whereas highest isolation rate (3.70%) was observed in Harnai breed for sheep Furthermore, statistically highly significant difference (Fisher's exact test P≤0.0001) in the isolation of Mycoplasma ovipneumoniae between healthy and respiratory distressed sheep breeds in Balochistan was found.

Authors and Affiliations

Shahid Amin| Institute of Microbiology University of Agriculture, Faisalbad, Pakitan, Department of Clinical Medicine and Surgery, University of Agriculture, Faisalbad, Pakitan, Sajjad ur Rahman| Institute of Microbiology University of Agriculture, Faisalbad, Pakitan, Department of Clinical Medicine and Surgery, University of Agriculture, Faisalbad, Pakitan, Iftikhar Hussain| Institute of Microbiology University of Agriculture, Faisalbad, Pakitan, Department of Clinical Medicine and Surgery, University of Agriculture, Faisalbad, Pakitan, Ghulam Muhammad| Institute of Microbiology University of Agriculture, Faisalbad, Pakitan, Department of Clinical Medicine and Surgery, University of Agriculture, Faisalbad, Pakitan, Mohammad Arif Awan| Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistan, Zafer Ahmad| Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistan, Mohammad Masood Tariq| Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistan, Muhammad Irfan Ullah| Department of Clinical Sciences Bahauddin Zakariya University Multan, Pakistan, Muhammad Azam Kakar| Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistan

Keywords

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  • EP ID EP13526
  • DOI 10.18869/IJABBR.2016.32
  • Views 367
  • Downloads 15

How To Cite

Shahid Amin, Sajjad ur Rahman, Iftikhar Hussain, Ghulam Muhammad, Mohammad Arif Awan, Zafer Ahmad, Mohammad Masood Tariq, Muhammad Irfan Ullah, Muhammad Azam Kakar (2016). Bimolecular identification of M.ovinpeumoniae from nasal swab samples of sheep from various districts of Balochistan, Pakistan. International journal of Advanced Biological and Biomedical Research, 4(1), 32-39. https://europub.co.uk/articles/-A-13526