Bio-Assay Guided Fractionation and Isolation of α-Glucosidase Inhibitory Constituents from Dillenia indica L. Fruit Extracts
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 17, Issue 2
Abstract
The plant Dillenia indica Linn. is used for the treatment of diabetes by the rural community of Dhemaji district of Assam, by the Khamptis “Chauya” traditional healers and Arunachal Himalaya, northeast, India. The plant is also reported to possess anti-diabetic and hypolipidemic activities in animal models. Based on the traditional uses and reported scientific contribution the plant was selected for the present investigation to study its α-glucosidase targeted action. The objective of the present study was to investigate the α-glucosidase inhibitory assay guided fractionation and isolation of phytoconstituents from D.Indicia fruit extracts. In the present investigation the methanolic fruit extract was prepared. The ethyl acetate fraction (EAF) from the methanol extract has shown a significant α-glucosidase enzyme inhibition activity (IC50 262.76 µg/ml). Total six constituents were isolated from different fractions and they were characterized and screened for α-glucosidase inhibition activity. Compound 2 (masilinic acid) was found to exert maximum IC50 (272.634 µg/ml) followed by compound 6 (IC50 669.8 µg/ml). Docking studies have revealed that masilinic acid binds through H-bonds to amino acid residues of α- glucosidase. Enzyme kinetics of masilinic acid has shown the probable chance of competitive binding to α-glucosidase. From the data it was concluded that the masilinic acid has exhibited the maximum inhibitory activity against α-glucosidase and it was the first report about the α-glucosidase inhibitory activity of D.Indica fruits.
Authors and Affiliations
Saiprasanna Rasamalla, Dr Kiranmai Mandava, Dr Pal BC, Sravanthi Madhira, Uma Rajeswari B, Dr Narendra Sharath Chandra JN
Keywords
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