Bio-Removal of Chlorophenols from Industrial Effluents in Open Bioreactor System
Journal Title: Journal of Chemistry and Applied Biochemistry - Year 2015, Vol 2, Issue 1
Abstract
Present study focused on the bio-removal of chlorophenols from the pulp and paper mill effluents which is a serious concern, for this work we used diluted black liquor containing 12 different chlorophenols and having high absorbable organic halogen (AOX), extractable organic halogen (EOX) and chemical oxygen demand (COD). Bio-removal of the chlorophenols was evaluated in open reactor system using consortium of five efficient bacterial strains isolated from the sludge of same industry, Gas chromatography (GC) analysis confirm that these isolate removed more than 95% of 12 different chlorophenols [2,4,6-trichlorophenol (2,4,6-TCP); 2,4,5-trichlorophenol (2,4,6-TCP); 2,3,4,5-tetrachlorophenol (2,3,4,5-TeCP); 3,4,6-trichloroguacol; 3,4,6-trichlorocatecol; 3,4,5-trichlorocatecol (3,4,5-TCP); Tetrachloroguaiacol; Tetrachlorocatecol; Trichlorosyringol; Pentachlorophenol (PCP); 3,4,5-trichloroguacol; 4,5,6-trichloroguacol] which are under regulation of environmental protection agency (EPA) in the wastewater to less than detection level,. Simultaneously AOX removal was 82%, EOX 65% and COD 92% was observed. Present result shows the high efficiency of the consortium for the pulp and paper mill effluents. Finally these isolate were identified using 16s rRNA gene sequencing which revealed that Rhodococcus sp. (SS1), Aeromonas sp. (SS2) Chryseobacterium sp. (SS3) Uncultured bacterium clones (SS4) and Pseudomonas sp. (SS5). Further this consortium was found to be noble and can be used for the removal of pollutants from the industrial effluents.
Authors and Affiliations
Santosh Kr. Karn
Keywords
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