BIOACTIVE POTENTIAL OF ENDOPHYTIC FUNGI ASPERGILLUS FLAVUS (SS03) AGAINST CLINICAL ISOLATES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 9
Abstract
Objective: This study was to evaluate the antimicrobial potential of endophytic fungi isolated from the medicinal plant Moringa oleifera Lam. collected from the Omalur region, Salem district.Methods: The endophytic fungi were isolated from stem, leaves, flowers and calyx of Moringa oleifera by surface sterilization method. The samples were surface sterilized by immersing it in 70% ethanol for 5 seconds followed by 4% sodium hypochlorite for 90 seconds and then a final rinsing in sterile distilled water. Then fungal biomass was extracted for intracellular metabolites by using ethyl acetate as solvent. The crude extract was filtered, and the filtrate was dried under vacuum at 40 °C. The filtrate was analyzed for antimicrobial activity. The fungi which showed the maximum activity was identified and the metabolite present in the ethyl acetate extract was characterized and identified by GC-MS and NMR analysis.Results: The predominant endophytic fungi isolated belongs to the genera of Aspergillus spp, Aspergillus flavus, Aspergillus versicolor, Aspergillus niger, Aspergillus ochraceus, Aspergillus terreus and dematiaceous fungi namely Bipolaris spp. From this Aspergillus flavus showed the highest zone of inhibition was observed against Staphylococcus aureus and Bacillus 22 mm and strain of Candida tropicalis 19 mm. The efficiency of the bioactive compound was identified by GC-MS and NMR analysis and found to be Fenaclon, (R)(-) 14 methyl-8-hexadecyn-1-ol, Trans-β-farnesene (E)-β-farnesene, 9-Octcadecene,1,1, DimethoxyConclusion: This study results indicate that the bioactive metabolites produce the endophytic fungi Aspergillus flavus could be promising source as antimicrobial agents.
Authors and Affiliations
Rajeswari S. , Umamaheswari S. , D. Arvind Prasanth, Rajamanikandan K. C. P.
Keywords
Related Articles
- EP ID EP576003
- DOI 10.22159/ijpps.2016.v8i9.11466
- Views 78
- Downloads 0
SYNTHESIS OF WOUND-HEALING KERATIN HYDROGELS USING CHICKEN FEATHERS PROTEINS AND ITS PROPERTIES
Objective: A novel cross-linked keratin hydrogel was prepared by integrating keratin from chicken feather into an aloe-vera, Chitosan and honey based dressing formulation separately.Methods: Keratin fibres extracted from...
DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF ASPIRIN, ROSUVASTATIN, CLOPIDOGREL IN BULK AND PHARMACEUTICAL DOSAGE FORM
Objective: To develop a novel, accurate, precise and linear reverse phase high-performance liquid chromatography (RP-HPLC) and Stability Indicating Assay Method (SIAMs) for simultaneous, qualitative and quantitative esti...
DEVELOPMENT AND VALIDATION OF ANALYTICAL SPECTROPHOTOMETRIC AND RP-HPLC METHODS FOR THE SIMULTANEOUS ESTIMATION OF HYDROQUINONE, HYDROCORTISONE AND TRETINOIN TERNARY MIXTURE IN TOPICAL FORMULATION
Objective: Development and validation of spectrophotometric and RP-HPLC methods for the simultaneous determination of Hydroquinone (HQ), Hydrocortisone (HC) and Tretinoin (TRT) ternary combination in pharmaceutical prepa...
EXPERIMENTAL AND COMPUTATIONAL STUDY OF BINDING INTERACTION OF ALKOXY DERIVATIVES OF N-ARYLHYDROXAMIC ACIDS WITH DNA
Objective: Â Binding affinity towards DNA for small molecules is very important in the development of new therapeutic reagents. Interaction between N-Arylhydroxamic acid derivatives N-p-Tolyl-4-Ethoxybenzohydroxamic acid...
ASSESSMENT OF GENETIC DIVERSITY IN TINOSPORA CORDIFOLIA BY INTER SIMPLE SEQUENCE REPEATS (ISSR) AND EXPRESSED SEQUENCE TAGGED- SIMPLE SEQUENCE REPEATS (EST-SSR)
Objective: In this study, assessment of genetic diversity was carried out using two kinds of molecular markers: Inter-Simple Sequence Repeats (ISSR) and Expressed Sequence Tag Simple Sequence Repeats (EST-SSR) in T. cord...