BIOANALYTICAL METHOD DEVELOPMENT FOR ESTIMATION OF DEFERASIROX IN HUMAN PLASMA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 9
Abstract
Objective: A rapid and selective high performance liquid chromatography (HPLC) method for estimation of Deferasirox in Human plasma was developed and validated.Methods: Analyte was recovered by protein precipitation technique and subsequently separated on C18 column (150x4.5 mm, 5µ) by using acetonitrile: phosphate buffer pH 3.0(60:40) as mobile phase at a flow rate of 1.0 ml/min at 248 nm.Results: The calibration curve was linear over the concentration range 0.4-4.8 µg/ml in human plasma. Within-batch and between-batch precision were<15% (at LLOQ<20%) and accuracy was all within 15%. (at LLOQ<20%).Conclusion: The developed and validated HPLC method was specific, sensitive and reproducible which can be used for routine drug analysis and bioanalysis.Â
Authors and Affiliations
M. S. Kalshetti, R. Y. Patil, R. A. Karale, A. A. Kulkarni
Keywords
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