BIOCHEMICAL CHANGES IN SERUM OF RABBITS WITH EXPERIMENTAL MECHANICAL DAMAGE OF THE BONE TISSUE AFTER APPLICATION OF ALLOGENEIC MESENCHYMAL STEM CELLS
Journal Title: Ukrainian Journal of Veterinary Sciences - Year 2018, Vol 1, Issue 285
Abstract
Summarizing the results of previous studies on the nature of the optimization and stimulation of reparative osteogenesis and the potential of studying this problem, our goal was to trace the biochemical changes during the process of reparative osteogenesis in experimental bone for the introduction of allogeneic mesenchymal stem cells. Damage of the bone tissue was modeled in rabbits 3 months of age of the chinchilla breed, in the middle third of the diaphysis of the tibia. Blood sampling was performed from the jugular vein before surgery and at 3, 7, 14, 21, 28 days after injury. Biochemical studies were made using biochemical analyzer RT-9600. The obtained results were processed statistically. The results of studies of biochemical changes, namely, content of Cа, P and alkaline phosphatase activity in blood serum of rabbits after an experimental mechanical damage of the bone tissue and the application of allogeneic mesenchymal stem cells. It was found that after the introduction of allogeneic mesenchymal stem cells for the regeneration of bone tissue, where the maximum increase in the content of Cа, P and alkaline phosphatase activity coming soon, soon comes back to normal compared to control group animals, starting with 3 day and ending on 28 day of the study. Which indicates that the stem cells is able to differentiate into osteogenic direction and influence the processes of bone tissue regeneration. And for the introduction of allogeneic mesenchymal stem cells in place of experimentally injured bone tissue regeneration processes are more intense in contrast to the introduced allogenic mesenchymal stem cells in the jugular. The obtained data can be used to develop methods and means of preventing possible complications in the recovery process of damaged bone tissue, as well as for further experimental studies.
Authors and Affiliations
T. L. Savchuk, A. I. Mazurkevych, M. O. Maliuk, Y. A. Kharkevich
Keywords
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