Biochemical Changes Induced by Gamma Irradiation in the Ground Beetle Blaps polycresta
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2016, Vol 9, Issue 3
Abstract
The exposure to low-dose gamma radiation from natural sources is a continuing and inescapable feature and it is common in certain occupations. The present study was designed to investigate the effects of low-dose gamma radiation on some detoxification enzymes such as; AST, ALT, ACP, ALP, GSH, GPX, SOD and CAT, as well as, its effect on total protein and DNA content in the soft tissues homogenate of males ground beetle Blaps polycresta. Insects were divided into three groups (group A, B and C) of 10 beetles each. Two groups (group B and C) were irradiated with doses at a rate of 32 Gy and 64 Gy; group A was considered as the control group. The study revealed a dose-dependent effect on the tested parameters. Significant increase in the enzyme activity of AST, ALT, ACP and ALP were observed in group B and C respectively compared to the control group (group A). The findings also showed a significant inhibitions in the enzymatic activity of GSH, GST, GPX, SOD and CAT in the irradiated groups compared to the control group. Furthermore, their radiated groups reported a decrease in protein and DNA content compared to the control group. Our observations suggested that the genotoxic effects of gamma radiation induced damaging effects on enzyme molecules, protein and nucleic acid (DNA) and may explain the increase in health risk from high doses of ionizing radiation.
Authors and Affiliations
Dalia A. Kheirallah, Lamia M. El-Samad
Keywords
Related Articles
- EP ID EP654567
- DOI 10.24297/jab.v9i3.1417
- Views 166
- Downloads 0
Microbial life of the deep saline biosphere
Transfer microbiological studies under the surface of the Earth to exploration of deep intraterrestrial microbial life is important to allows exceeding the current framework of science. Microbial life is determined by ph...
Laminaria Japonica polysaccharide promotes the expression of calcitonin receptor-like receptor in type 2 diabetes mellitus model mice
Amylin, or islet amyloid polypeptide (IAPP), is a 37-residue peptide hormone[1-2]. It is cosecreted with insulin from the pancreatic β cells in the ratio of approximatrly 100:1[3-4]. Amylin receptor (AR) is compos...
VISUAL SEXUAL STIMULATION THROUGH THE AMYGDALA MAY AFFECT THE INDIVIDUALS EMOTIONAL STATE
The nature of sexual stimulation in the brain is not well established. In this regard, the role of emotional brain (which is called as the amygdaloid complex) needs to be evaluated more precisely. The amygdala is a silen...
ISOLATION, CHARACTERISTICS, AND ANTIOXIDANT ACTIVITY OF LOW MOLECULAR COMPOUNDS OF FRUIT BODIES LACTARIUS PERGAMENUS (Fr.) Fr MUSHROOMS
Methanol and methylene chloride extracts were obtained from fresh and dried fruit bodies of Lactarius pergamenus (Fr.) Fr. and studied by gas-chromatography mass spectrometry (GC/MS), thin-layer chromatography (TLC), and...
Neopterin-inhibitor of dihydrofolate reductase and hypothetical action in the human body
Concentration of neopterin in the organism increases during many diseases and therefore it is used as a marker to assess the severity of these disease, applied treatment severity, oxidative stress and the cellular immune...