Biochemical Identification of Aggregatibacter actinomycetemcomitans in an Indian Sample with Aggressive Periodontitis
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 7, Issue 1
Abstract
Background: The role of microorganisms and the etiology of periodontal disease is well established. Despite the fact that periodontal diseases are caused by dental plaque there existed some controversies regarding the role of specific organisms in the pathogenesis of the periodontal disease. This problem was overcome by the specific plaque hypothesis. Generally, the periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa) is identified employing anaerobic culture and in recent years through polymerized chain reaction. Very few attempts have been done to establish the confirmation of Aa through biochemical reactions. Hence this study was under taken to confirm Aa from the plaque sample by various biochemical tests. Aim of the Study: Biochemical identification of Aggregatibacter actinomycetemcomitans, a specific periodontal pathogen from the plaque samples of aggressive periodontitis patients. Materials and Methods: A total of forty patients 24 males and 16 females in the age range of 18-25 yrs. Diagnosed as aggressive periodontitis were included in this study. Plaque samples were collected from the periodontal pockets of aggressive periodontitis patients and were subjected to various biochemical tests. Results: Biochemical tests confirmed that the periodontal pathogen collected from the plaque samples were Aggregatibacter actinomycetemcomitans. Conclusion: Biochemical tests can be used as a viable economic alternative for the confirmation of the periodontal pathogen Aa.
Authors and Affiliations
R. Saranyan, B. Manovijay, G. Balaji Babu, Rajasekar . , I. Nithya, A. Anitha
Keywords
Related Articles
- EP ID EP338277
- DOI 10.9734/JAMB/2017/37877
- Views 84
- Downloads 0
Isolation and Purification of an Antifungal Protein from Kiwi Fruits and Demonstration of Its Antifungal Activity
A single polypeptide low molecular weight 25 KDa thaumatin-like antifungal protein was purified from Hayward variety of kiwifruits. It was purified to apparent homogeneity involving saline extraction, (NH4)2SO4 precipita...
The Antimicrobial Effects of Secondary Metabolites of Anguillan Fungi
Introduction: Many drugs have been isolated from fungal species. This study aims at identifying fungal species isolated in Anguilla in order to determine the antimicrobial effect of their secondary metabolites from pure...
pH Effect and pH Changes during Biocellulose Production by Gluconacetobacter xylinus in Moringa oleifera Tea-Sugar Medium
The study was carried out to determine the effect of pH and pH changes accompanying Biocellulose (BC) production by Gluconacetobacter xylinus (GX) in a Moringa oleifera tea-sugar medium. The authors adopted experimental...
Multidrug Resistance, V16S rRNA Gene Sequencing, High Frequency of Toxins Encoding Genes (tox, tdh, trh) in Parahaemolyticus and Non -parahaemolyticus Vibrios Isolated from Shellfish
PCR amplification of V16S-rRNA, tox, tdh, trh was applied as a molecular typing approach to identify potentially toxigenic vibrio species. Thirty vibrio strains were isolated from several types of shellfish collected fro...
Prevalence of Listeria monocytogenes in Fresh and Raw Fish, Chicken and Beef
There has been recent outbreaks of food poisoning associated with Listeria monocytogenes. Most of the outbreaks were attributed to fresh meat and dairy products, and processed foods. This study was therefore carried out...