Biodegradation of total petroleum hydrocarbons in contaminated soils using indigenous bacterial consortium
Journal Title: Environmental Health Engineering and Management Journal - Year 2020, Vol 7, Issue 2
Abstract
Background: Biodegradation of hydrocarbon compounds is a great environmental concern due to their toxic nature and ubiquitous occurrence. In this study, biodegradation potential of oily soils was investigated in an oil field using indigenous bacterial consortium. Methods: The bacterial strains present in the contaminated and non-contaminated soils were identified via DNA extraction using 16S rDNA gene sequencing during six months. Furthermore, total petroleum hydrocarbons (TPH) were removed from oil-contaminated soils. The TPH values were determined using a gas chromatograph equipped with a flame ionization detector (GC-FID). Results: The bacterial consortium identified in oil-contaminated soils (case) belonged to the families Halomonadaceae (91.5%) and Bacillaceae (8.5%), which was significantly different from those identified in non-contaminated soils (control) belonging to the families Enterobacteriaceae (84.6%), Paenibacillaceae (6%), and Bacillaceae (9.4%). It was revealed that the diversity of bacterial strains was less in oil-contaminated soils and varied significantly between case and control samples. Indigenous bacterial consortium was used in oil-contaminated soils without need for amplification of heterogeneous bacteria and the results showed that the identified bacterial strains could be introduced as a sufficient consortium for biodegradation of oil-contaminated soils with similar texture, which is one of the innovative aspects of this research. Conclusion: An oil-contaminated soil sample with TPH concentration of 1640 mg/kg was subjected to bioremediation during 6 months using indigenous bacterial consortium and a TPH removal efficiency of 28.1% was obtained.
Authors and Affiliations
Yalda Basim , Ghasemali Mohebali , Sahand Jorfi , Ramin Nabizadeh , Mehdi Ahmadi Moghadam , Ata Ghadiri , Nematollah Jaafarzadeh Haghighi Fard
Keywords
Related Articles
- EP ID EP685194
- DOI -
- Views 194
- Downloads 0
Investigating the reduction of BTEX in automotive paint sludge combined with biological sludge by vermicomposting process using Eisenia fetida
Background: Due to the fact that in the process of car painting in the automotive industry, sludge containing dangerous compounds of benzene, toluene, ethylbenzene, xylene which cannot be released into the environment...
Single and competitive adsorption of Cu2+ and Pb2+ by tea pulp from aqueous solutions
Background: Removal of heavy metals by bioadsorbents is one of the effective and inexpensive methods for water and wastewater treatment. The aim of this study was to investigate the ability of tea pulp in order to remove...
Effects of operational parameters on methyl tert-butyl ether removal by permeable reactive barrier from polluted waters
Background: Recalcitrant organics remediation from water resources continues to be a significant environmental problem and there is a continued effort to demonstrate practicable and economical treatment options for pol...
Removal of Acid Orange 7 dye from wastewater using combination of ultraviolet radiation, ultrasonic method, and MgO nanoparticles
Background: Industrial dyes are toxic and carcinogenic, therefore, they should be removed from wastewater. The aim of this study was to investigate the removal of acid orange 7 Dye from wastewater using ultraviolet (UV)...
Comparison ability of algae and nanoparticles on nitrate and phosphate removal from aquaculture wastewater
Background: Aquaculture wastewater contains high levels of phosphate and nitrate. The reuse of this water requires standards beyond the secondary standards to eliminate more organic pollutants from aquaculture effluents....