BIOFILM FORMATION BY PSEUDOMONAS AERUGINOSA STRAINS WITH DIFFERENT LEVEL OF THE INTRACELLULAR C-DI-GMP IN PRESENCE OF SIGNAL QUINOLON SYNTHETIC ANALOGS
Journal Title: Мікробіологія і біотехнологія - Year 2018, Vol 0, Issue 2
Abstract
Aim. Study of the biofilm formation of Pseudomonas aeruginosa cells with different level of intracellular cyclic-3,5-diguanosinmonophosfate (c-di-GMP) in presence of original derivatives of 2-heptyl-3-hidroxy-4-quinolon with different length of alkyl chain. Methods. Test strain cells were incubated in 96-well plates in presence 20, 40 and 80 µM of discovered compounds. The compounds were divided in to two groups – with shorter length of alkyl chain then in PQS, and with longer length of alkyl chain then in PQS. Planktonic culture growth was determined spectrophotometricly on the wave length 600 nm. Biofilm formation was studded by CV-test (crystal violet-test) spectrophotometricly on the wave length 592 nm. Results. The obtained results showed that PQS analogs with shorter length of alkyl chain in most cases slightly decrease planktonic cells content. In the other hand, the derivatives with longer length of alkyl chain showed more sufficient effects on planktonic culture content. PQS and its synthetic analogs increased biofilm mass of P. aeruginosa PA01 and P. aeruginosa PA01 pJN2133 on 34–79% and 135–217%, respectively. In the case of P. aeruginosa PA01 ΔwspF1 the discovered compounds showed opposite effect – the decreased biofilm mass in 1.4–2 times compare to the control. Conclusions. It has been shown that PQS and its synthetic derivatives with different alkyl chain length modulate biofilm formation process in P. aeruginosа depending on intracellular level of c-di-GMP: stimulate in the strains with natural and decreased amount but inhibit in the case of high amount of this secondary messenger.
Authors and Affiliations
M. B. Galkin, S. V. Vodzinsky, L. М. Strezeva, М. А. Dzhura, Т. О. Filippova
Keywords
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- EP ID EP396610
- DOI 10.18524/2307-4663.2018.2(42).134716
- Views 112
- Downloads 0
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