Biofilm formation capability of bacteria, a threat to treat multi drug resistant diabetic foot infection isolates
Journal Title: Pakistan Journal of Pharmaceutical Research - Year 2017, Vol 3, Issue 2
Abstract
Bacterial biofilms are complex structured communities surrounded in a matrix of extra-cellular polymeric substances growing on rough surfaces. It is involved in multiple functions like trapping of nutrients, adherence, stability of biofilm structure and the penetration of many antibiotics.250Pus samples were collected from the depth of ulcers from each patient using Amies Transport Swabs. The specimens were inoculated on appropriate media. Biochemical characteristics of pathogens were observed by performing conventional biochemical tests and Quick Test Strips. Antibiotic resistance of isolates was checked by Kirby Bauer’s Disc Diffusion Method. Biofilm formation was initially detected by Congo Red Agar Method. Major biofilm forming isolates from different hospitals environment were Klebsiella spp., Corynebacterium spp., S. epidermidis while from hospital curtain’s samples, Shigella dysenteriae, Candida albicans and Aspergillus flavus were having capability of biofilm formation. S. aureus showed good biofilm forming ability in presence of 0.2% glucose, sucrose and lactose, P. aeruginosa showed enhancement in biofilm formation in presence of sucrose, glucose, and maltose while E. coli indicated increased biofilm formation in presence of lactose, sucrose and glucose. Most of the biofilm forming isolates were Multi drug resistant which indicate that extracellular material released by these pathogens cover the bacteria cell and hinder the antibiotic
Authors and Affiliations
Mubashar Aziz
Keywords
Related Articles
- EP ID EP511563
- DOI -
- Views 105
- Downloads 0
Evaluation of inorganic elements in herbal preparations by FAAS and FES
In this study four inorganic elements i.e. sodium, potassium, calcium and magnesium were estimated in eleven locally available herbal preparations used as sex stimulants using Flame Atomic Absorption Spectrometry and Fla...
Biofilm formation capability of bacteria, a threat to treat multi drug resistant diabetic foot infection isolates
Bacterial biofilms are complex structured communities surrounded in a matrix of extra-cellular polymeric substances growing on rough surfaces. It is involved in multiple functions like trapping of nutrients, adherence, s...
Opinion about health issues regarding Transgenic Farm animals
For the betterment of mankind, scientists are trying to overcome all factors that hinder our economy and health. For this purpose they form genetically modified animals or organisms that have required characteristics, im...
Gender specific correlation among fingerprint patterns and blood group in Pakistani population
Finger print patterns are extensively used for the study of individuality as most legitimate evidence against criminals. Due to the massive potential of fingerprints as a definite method of identification they can be use...
Survey of socio-demographic prevalence, risk factors and clinical characterization of tuberculosis in Nishtar Hospital Multan
Tuberculosis is a worldwide health problem affecting one third of world’s population and Pakistan is no exception. In this study, we used a structured and close-ended questionnaire to collect data about sociodemographic...