Biofilm from patients with chronic rhinosinusitis. Morphological SEM studies
Journal Title: Otolaryngologia Polska - Year 2008, Vol 62, Issue 3
Abstract
Introduction. Bacterial biofilm is a three-dimensional structure made of aggregates of bacterial cells (microcolonies) and the extra cellular matrix released by them, adhering to organic and inorganic surfaces. It is estimated that 99% of all bacteria exist in biofilms, and only 1% live in a free-floating or planktonic state at any given time. The aim of the study was to demonstrate biofilms in mucosal specimens of patients undergoing endoscopic sinus surgery for chronic rhinosinusitis and co-occurrence of such illnesses as nasal polyps, bronchial asthma, NSAIDs allergy, and aspirin-induced asthma. Material and methods. A prospective study of 25 patients suffering from chronic rhinosinusitis. All patients underwent completed a otolaryngological examination and paranasal sinus CT scans. Endoscopic surgery of nasal sinuses (ESS) was performed in all patients. The mucous membrane samples were taken from the inside of the sinus and concha bullosa. They were prepared and examined with a scanning electron microscope (SEM). The images were then compared with the available database of the biofilm images. Results. Using SEM morphologic criteria, the biofilms were identified in the studied material in nineteen patients (83%). In four patients with chronic rhinosinusitis, no biofi lms were found on the samples taken. A bacteriological examination of smears from inside of the sinus, revealed the presence of various types of bacteria. In two patients no bacteria was found in the smears. Conclusions. It is supposed that bacterial biofilms perform an essential role in the pathogenesis of chronic sinusitis. One may speculate that, the presence of biofilms in patients with chronic rhinosinusitis could induce the co-occurrence of such illnesses as nasal polyps, bronchial asthma, aspirin-induced asthma or a NSAIDs allergy. In order to evaluate better the role of biofi lms in chronic rhinosinusitis, it seems justified to extend the research to a larger group of patients and a control group.
Authors and Affiliations
Roman Głowacki, Paweł Stręk, Katarzyna Zagórska-Świeży, Jacek Składzień, Krzysztof Oleś, Karolina Hydzik-Sobocińska, Adam Miodoński
Keywords
Related Articles
- EP ID EP51495
- DOI -
- Views 97
- Downloads 0
Regulacje molekularne procesu niszczenia kości w zapaleniu perlakowym ucha
Bone destruction in chronic otitis media with cholesteatoma is a common phenomenon. Expanding growth of cholesteatoma in the middle ear causes ischemia of the mucosa and bones with granulation tissue production.The aim o...
Sprawozdanie z 46 Kongresu Południowo - Afrykańskiego Towarzystwa Otorynolaryngologii Chirurgii Głowy i Szyi oraz Południowo-Afrykańskiego Stowarzyszenia Audiologów i Południowo Afrykańskiego -Towarzystwa Onkologii Głowy i Szyi
W dniach od 24 do 27 października 2010 r. odbył się 46. Kongres Południowo-Afrykańskiego Towarzystwa Otorynolaryngologii Chirurgii Głowy i Szyi (South Afri-can Society of Otorhinolaryngology Head Neck Surgery) we współpr...
Przerzut gruczolakoraka odbytnicy do skóry twarzy – opis przypadku
Introduction. Carcinoma of the large intestine is a common and relatively well known neoplastic malignancy of the digestive tract. In a very few cases its spread seems unpredictable and can cause a distant metastasis to...
Percepcja smaku u chorych z przewlekłym zapaleniem zatok przynosowych z polipami nosa leczonych doustną glikokortykosteroidoterapią
Background: Chronic rhinosinusitis with nasal polyps is observed in 1–4% persons during rhinoscopy. While the occurrence of their problems is connected with smell sense, still not enough attention is paid to the malfunct...
Przydatność arytenoidektomii laserowej i laterofiksacji w leczeniu obustronnego porażenia fałdów głosowych