Bioflm Production in Carbapenem Resistant Isolates from Chronic Wound Infections
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2017, Vol 6, Issue 2
Abstract
Bioflms are communities of microorganisms covered with extracellular polymeric substances. Such bioflm phenotype makes the microorganism resistant to antibiotics and plays a role in wound chronicity. This results in prolonged hospital stays in ICU, greater cost, and increased mortality. Methods: Pus swabs (59) were collected from a tertiary care hospital near Chennai were processed and identifed using standard procedure followed by antibiotic susceptibility testing and identifcation of carbapenem resistance by Modifed Hodge test as per CLSI guidelines. The bioflm formation was tested using plastic microtiter plate method. Results: Out of 59 pus swabs, 51 yielded growth with 69 isolates and 8 yielded no growth. Among the 69 isolates, 51 were GNB and 18 were GPC. Bioflm detection was noted in 84.31% (43/51) GNB isolates with 0.1% crystal violet whereas 100% (51/51) showed bioflm positive with 0.1% safranin. About 74.50% (38/51) isolates of GNB were carbapenem resistant by screening with disk diffusion method. Only 24% (6/25) of GNB isolates among Enterobacteriaceae were positive by Modifed Hodge test method. Conclusion: The result shows the association of bioflm production among carbapenem resistant isolates obtained from chronic wound infections.
Authors and Affiliations
Swarna SR| Department of Microbiology, JIPMER, Karaikal, Puducherry, India, Corresponding e-mail: srswa_20@yahoo.co.in, Swedha| Department of Microbiology, SRMMCH & RC, SRM University, SRM Nagar, Kattankulathur, Kancheepuram, Tamil Nadu, India, Gomathi S| Department of Microbiology, SRMMCH & RC, SRM University, SRM Nagar, Kattankulathur, Kancheepuram, Tamil Nadu, India
Keywords
Related Articles
- EP ID EP12315
- DOI -
- Views 297
- Downloads 15
Influence of MAP and Multi-layer Flexible Pouches on Clostridium Count of Smoked Kutum Fish (Rutilus frisii kutum)
In this study the effect of different concentrations of three gas mixture (carbon dioxide, nitrogen, oxygen), and also vacuum conditions and flexible multi-layer films were evaluated on Clostridium count of smoked kutum...
A PROFILE OF CARDIOVASCULAR DISEASES IN A TEACHING HOSPITAL IN KERALA
Background and objective: cardiovascular diseases are increased in each year in India. Cardiovascular diseases more are occurred in the economically productive age group. This will affect their family and also the natio...
COMPARATIVE EVALUATION OF AZELASTINE AND FLUOROMETHOLONE IN THE TREATMENT OF SPRING CATARRH (VERNAL CATARRH)
Aim and Objective: To study and compare the efficacy of FML &Azelastin eye drops in relieving symptoms & regressing signs of VKC. Methodology: This Prospective Interventional study conducted at Sarojini Devi eye hospit...
LIVED EXPERIENCES OF HEALTH PROBLEMS OF ELDERLY RESIDING IN URBAN AREAS, KATHMANDU: PILOT STUDY
Introduction: Globally, number of old age population is increasing with advancement of biomedical technology. Old age is the time associated with biological, psychological and social changes which situate elderly to ac...
ASSOCIATION BETWEEN REFRACTIVE ERRORS AND SENILE CATARACT IN RURAL AREA OF WESTERN MAHARASHTRA
Purpose: To study the association between refractive errors and senile cataract in rural area of western MaharashtraMaterials & Methods: It is a prospective cross sectional study carried out on 420 eyes of 210 patients...