Bioflm Production in Carbapenem Resistant Isolates from Chronic Wound Infections

Abstract

Bioflms are communities of microorganisms covered with extracellular polymeric substances. Such bioflm phenotype makes the microorganism resistant to antibiotics and plays a role in wound chronicity. This results in prolonged hospital stays in ICU, greater cost, and increased mortality. Methods: Pus swabs (59) were collected from a tertiary care hospital near Chennai were processed and identifed using standard procedure followed by antibiotic susceptibility testing and identifcation of carbapenem resistance by Modifed Hodge test as per CLSI guidelines. The bioflm formation was tested using plastic microtiter plate method. Results: Out of 59 pus swabs, 51 yielded growth with 69 isolates and 8 yielded no growth. Among the 69 isolates, 51 were GNB and 18 were GPC. Bioflm detection was noted in 84.31% (43/51) GNB isolates with 0.1% crystal violet whereas 100% (51/51) showed bioflm positive with 0.1% safranin. About 74.50% (38/51) isolates of GNB were carbapenem resistant by screening with disk diffusion method. Only 24% (6/25) of GNB isolates among Enterobacteriaceae were positive by Modifed Hodge test method. Conclusion: The result shows the association of bioflm production among carbapenem resistant isolates obtained from chronic wound infections.

Authors and Affiliations

Swarna SR| Department of Microbiology, JIPMER, Karaikal, Puducherry, India, Corresponding e-mail: srswa_20@yahoo.co.in, Swedha| Department of Microbiology, SRMMCH & RC, SRM University, SRM Nagar, Kattankulathur, Kancheepuram, Tamil Nadu, India, Gomathi S| Department of Microbiology, SRMMCH & RC, SRM University, SRM Nagar, Kattankulathur, Kancheepuram, Tamil Nadu, India

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  • EP ID EP12315
  • DOI -
  • Views 278
  • Downloads 15

How To Cite

Swarna SR, Swedha, Gomathi S (2017). Bioflm Production in Carbapenem Resistant Isolates from Chronic Wound Infections. International Journal of Medical Research & Health Sciences (IJMRHS), 6(2), 61-67. https://europub.co.uk/articles/-A-12315