Bioguided extraction and Evaluation of Antioxidant studies of Abutilon indicum fruits
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2016, Vol 5, Issue 6
Abstract
The present study was conducted to evaluate the antioxidant activity, to determine the phytoconstituents, phenolic content and flavonoids in different fractions of Abutilon indicum fruits. The hydro alcoholic extract of Abutilon indicum fruits was fractionated with organic solvents with increasing polarity. In vitro antioxidant activity was assayed by diphenyl picryl hydrazyl radical scavenging assay, nitric oxide radical scavenging assay, superoxide radical scavenging assay, hydroxyl radical scavenging assay, ferric-reducing power assay. Phenolic content was determined by folin ciocalteu reagent method. Among all fractions the ethyl acetate fraction was found to possess good antioxidant activity, followed by chloroform fraction. The total phenolic (86 mg gallic acid equivalent/g), flavonoid (30 mg rutin equivalent/g) content in ethyl acetate fraction is high when compared to other fractions. These results reveal that ethyl acetate fraction of A. indicum fruits has strong antioxidant potential compared with all other fractions. Further studies are necessary for isolation and characterization of active constituent. Key words: Abutilon indicum, fractions, folin ciocalteu reagent, antioxidant, flavonoid content.
Authors and Affiliations
Rajesh Bolleddu
Keywords
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