Biological activities of Barleria cristata
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2013, Vol 2, Issue 2
Abstract
The methanol extract of leaves of Barleria cristata L. as well as its hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screening for antioxidant, cytotoxic, thrombolytic, membrane stabilizing and antimicrobial activities. The antioxidant potential was evaluated by DPPH and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. The extractives of B. cristata exhibited weak free radical scavenging activity. The carbon tetrachloride soluble fraction exhibited an IC50 value of 300.82±0.33 μg/ml which demonstrated total phenolic content of 89.22±0.12 mg of GAE / g of extractives. The general toxicity was determined by brine shrimp lethality bioassay where the hexane (LC50 =1.52±0.34 μg/ml) soluble fraction revealed the presence of considerable bioactive principles. Mild thrombolytic activity was discerned by B. cristata extractives. During assay for thrombolytic activity, the aqueous soluble fraction exhibited 45.0±0.22 % of clot lysis as compared to 65.66% and 3.791% clot lysis by standard streptokinase and water, used as positive and negative controls, respectively. The crude methanol extract and chloroform soluble fraction inhibited 55.36±0.12% & 35.7±0.05% and 48.95±0.02% & 21.77±0.87% haemolysis of RBCs induced by hypotonic solution and heat, respectively. Here, acetyl salicylic acid (0.1 mg/ml) was used as reference showing 71.9% and 42.12% inhibition of haemolysis of RBCs in hypotonic solution and heat induced conditions, respectively. The chloroform soluble materials demonstrated activity against microbial growth with zone of inhibition ranging from 7.0 to 26.0 mm.
Authors and Affiliations
Tasnuva Sharmin
Keywords
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